Analysis of urinary 8-hydroxy-2´-deoxyguanosine by high-performance liquid chromatography with electrochemical detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49813994%3A_____%2F09%3A%230000016" target="_blank" >RIV/49813994:_____/09:#0000016 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Analysis of urinary 8-hydroxy-2´-deoxyguanosine by high-performance liquid chromatography with electrochemical detection
Original language description
8-hydroxy-2?-deoxyguanosine (8-OH-2?-dG) is one of the predominant forms of radical-induced lesions to DNA, and therefore it has been widely used as a biomarker for oxidative stress. We studied an analytical procedure of determination of urinary 8-OH-2?-dG. A routine assay of free 8-OH-2?-dG in body fluids such as urine is problematic, therefore the purification of this adduct from urine employs solid-phase extraction (SPE) procedure. Various SPE columns from different producers were used as follows: Waters ? Oasis MCX (sorbent silica + benzensulfonic acid), Supelco Discovery C18 Oktadecyl (sorbent silica + C18), UCT Columns ? Clean Screen DAU206 (sorbent silica + C18 + benzenculfonic acid) and Styre Screen DBX (sorbent styrene-divinylbenzene + C18 + benzensulfonic acid), Macherey-Nagel Chromabond HR-X (sorbent styrene-divinylbenzene) and HR-X-C (sorbent styrene-divinylbenzene + benzensulfonic acid), Phenomenex STRATA X-C (sorbent styrene-divinylbenzene + benzensulfonic acid).
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Result continuities
Project
<a href="/en/project/KAN208130801" target="_blank" >KAN208130801: New design and exploiting nanobiosensors and nanosensors to target medicine (NANOSEMED)</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
International Conference VITAMINS, NUTRITION, DIAGNOSTIC Brno, Czech Republic, August 31 - September 2, 2009 The Abstract Book
ISBN
978-80-7318-809-2
ISSN
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e-ISSN
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Number of pages
2
Pages from-to
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Publisher name
Univerzita Tomáše Bati ve Zlíně
Place of publication
nám. T. G. Masaryka 5555, 760 01 Zlín
Event location
Brno
Event date
Aug 31, 2009
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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