Determination of quercetin glycosides and free quercetin in buckwheat by capillary micellar electrokinetic chromatography

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F10%3A00012481" target="_blank" >RIV/60076658:12220/10:00012481 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Determination of quercetin glycosides and free quercetin in buckwheat by capillary micellar electrokinetic chromatography

Original language description

The simple and reliable method for determination of quercetin glycosides and free quercetin in buckwheat flower, leaves, stems and achenes was developed. The method consists of flavonoid extraction from freeze-dried homogenous material in 50% (v/v) methanol solution and in presence of an antioxidant, cleaning of extract and analyte isolation using solid phase extraction. Analytical step uses capillary micellar electrokinetic chromatography. The working ranges, limit of detection and quantification, recovery, precision and measurement uncertainty were calculated. The method is suitable for samples from buckwheat. The highest content of rutin was found in flowers of both kinds of buckwheat (99 400 mg?kg-1 in F. esculentum, 108 000 99 400 mg?kg-1 in F. tataricum). The free quercetin occurs in flowers and achenes of F. esculentum, while flowers and achenes of F. tataricum contained quercitrin.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Volume of the periodical

33

Issue of the periodical within the volume

11

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

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UT code for WoS article

000278829700014

EID of the result in the Scopus database

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