Fusarium Identification by PCR and DON Content in Grain of Ancient Wheat

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F11%3A43882059" target="_blank" >RIV/60076658:12220/11:43882059 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Fusarium Identification by PCR and DON Content in Grain of Ancient Wheat
Original language description
Fusarium infestation and DON content were studied in genetic resources of spring wheat (einkorn, emmer wheat, spelt wheat, intermediate forms of bread wheat). The study aimed at the comparison of grain contamination rates in various wheat species being grown in organic farming systems. The trials were established on certified organic parcels in two different localities in the Czech Republic in 2009 and 2010. The PCR method and specific primers were used to detect Fusarium. The proportion of deoxynivalenol (DON) was indicated and measured by the immuno-affinity chromatography (ROSA(R) DON Quantitative Test). The grain DON contamination rate (1.25 mg/kg) was set up in compliance with the European Commission (EC) Regulation No. 1126/2007. This regulationsets up limits that have never been exceeded in any of the varieties. The strongest contamination rate was identified in grains of the control bread wheat varieties, and in the SW Kadrilj cultivar (0.98 mg/kg) being grown in Ceske Budejov
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GC - Plant growing, crop rotation
OECD FORD branch
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Project
<a href="/en/project/QH82272" target="_blank" >QH82272: Utilization of spring forms of selected wheat varieties in organic farming.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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