Occurrence and genetic diversity of Cryptosporidium spp. in wild foxes, wolves, jackals, and bears in central Europe

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F21%3A43903270" target="_blank" >RIV/60076658:12220/21:43903270 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/21:43903270 RIV/60077344:_____/21:00554243 RIV/60460709:41320/21:85465

Result on the web

<a href="http://folia.paru.cas.cz/pdfs/fol/2021/01/02.pdf" target="_blank" >http://folia.paru.cas.cz/pdfs/fol/2021/01/02.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14411/fp.2021.002" target="_blank" >10.14411/fp.2021.002</a>

Result language

angličtina

Original language name

Occurrence and genetic diversity of Cryptosporidium spp. in wild foxes, wolves, jackals, and bears in central Europe

Original language description

Parasites of the genus Cryptosporidium Tyzzer, 1910 are one of the most common protistan parasites of vertebrates. Faecal samples from 179 red foxes (Vulpes vulpes [Linnaeus]), 100 grey wolves (Canis lupus Linnaeus), 11 golden jackals (Canis aureus Linnaeus), and 63 brown bears (Ursus arctos Linnaeus) were collected in the Czech Republic, Poland and Slovakia. Samples were examined for the presence of Cryptosporidium spp. using microscopy and PCR/sequence analysis. Phylogenetic analysis based on the small subunit ribosomal RNA (SSU), actin and 60-kDa glycoprotein (gp60) genes using the maximum likelihood method revealed the presence of Cryptosporidium tyzzeri Ren, Zhao, Zhang, Ning, Jian et al., 2012 (n = 1) and C. andersoni Lindsay, Upton, Owens, Morgan, Mead et Blackburn, 2000 (n = 2) in red foxes, C. canis Fayer, Trout, Xiao, Morgan, Lai et Dubey, 2001 (n = 2) and C. ubiquitum Fayer, Santin et Macarisin, 2010 (n = 2) in grey wolves, and C. galli Pavlasek, 1999 in brown bears (n = 1) and red foxes (n = 1). Subtyping of isolates of C. ubiquitum and C. tyzzeri based on sequence analysis of gp60 showed that they belong to the XIId and IXa families, respectively. The presence of specific DNA of C. tyzzeri, C. andersoni and C. galli, which primarily infect the prey of carnivores, is probably the result of their passage through the gastrointestinal tract of the carnivores. Finding C. ubiquitum XIId in wolves may mean broadening the host spectrum of this subtype, but it remains possible this is the result of infected prey passing through the wolf - in this case deer, which is a common host of this parasite. The dog genotype of C. canis was reported for the first time in wolves.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10613 - Zoology

Project

<a href="/en/project/LTAUSA17165" target="_blank" >LTAUSA17165: Diversity and co-evolution of Cryptosporidium parasiting in rodents: Linking genetic variation to parasite biology</a><br>

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Parasitologica

ISSN

0015-5683

e-ISSN

—

Volume of the periodical

68

Issue of the periodical within the volume

FEB 2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

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UT code for WoS article

000624328400001

EID of the result in the Scopus database

2-s2.0-85101033425