Development of loop mediated isothermal amplification for rapid species detection of Armillaria ostoyae using assimilating probe.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F22%3A43905868" target="_blank" >RIV/60076658:12220/22:43905868 - isvavai.cz</a>

Result on the web

<a href="https://jfs.agriculturejournals.cz/artkey/jfs-202205-0001_development-of-loop-mediated-isothermal-amplification-for-rapid-species-detection-of-armillaria-ostoyae-using-a.php" target="_blank" >https://jfs.agriculturejournals.cz/artkey/jfs-202205-0001_development-of-loop-mediated-isothermal-amplification-for-rapid-species-detection-of-armillaria-ostoyae-using-a.php</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/161/2021-JFS" target="_blank" >10.17221/161/2021-JFS</a>

Result language

angličtina

Original language name

Development of loop mediated isothermal amplification for rapid species detection of Armillaria ostoyae using assimilating probe.

Original language description

We introduced here the first loop mediated isothermal amplification (LAMP) assay for the identification of honey fungus, Armillaria ostoyae, a basidiomycote playing an important role in spruce declines in the Palaearctic region. In total, 101 isolates, representing three Armillaria species, were used to develop a new LAMP assay to determine species specific identification. We have here described LAMP primers enhanced with fluorescent dye that are able to amplify A. ostoyae DNA and detect fungi in a fast single step reaction. The detection limit of LAMP was 1 pg of genomic DNA per reaction. We optimized a new LAMP assay for the rapid detection of A. ostoyae using the translation elongation factor 1-α (tef1) marker and fluorescence labelled oligonucleotide assimilating probe. The LAMP assay does not require any specialized equipment, hence it can be used in the field for the rapid detection of A. ostoyae even using the portable and mobile device. The specificity of the assay was confirmed by the use of A. ostoyae strains and Armillaria cepistipes and Armillaria gallica strains, respectively. In conclusion, the assay could be a rapid, specific, sensitive and low-cost tool for identification of A. ostoyae as well as the first step for expansion of this method in practical applications.

Czech name

—

Czech description

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Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

—

OECD FORD branch

10612 - Mycology

Project

<a href="/en/project/QK1920412" target="_blank" >QK1920412: Mycoviruses as potential agents in the protection of spruce stands against honey fungus</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Forest Science

ISSN

1212-4834

e-ISSN

—

Volume of the periodical

68

Issue of the periodical within the volume

5

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

163-169

UT code for WoS article

000800595600001

EID of the result in the Scopus database

2-s2.0-85131895605