The introduction of RT-LAMP method and comparison of its sensitivity with RT-qPCR in the detection of Chikungunya virus

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70565813%3A_____%2F13%3A%230000291" target="_blank" >RIV/70565813:_____/13:#0000291 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
The introduction of RT-LAMP method and comparison of its sensitivity with RT-qPCR in the detection of Chikungunya virus
Original language description
Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method and has the potential to compete with PCR because of its simplicity, rapidity, specificity, and cost-effectiveness. The reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay has emerged as a powerful gene amplification tool for the rapid identification of microbial infections and is being increasingly used for rapid detection and typing of emerging viruses, for example the Chikungunya virus,West Nile virus, dengue virus etc. The detection of gene amplification can be accomplished by either agarose gel electrophoresis or by real-time monitoring in the turbidimeter. Moreover, the gene amplifification can be eye-visualized either by formationof the white precipitate or by using fluorescent intercalating dye (e.g. SYTO) and UV lamp. Because the LAMP method uses a Bst DNA polymerase long fragment, the reaction can be carried out under isothermal conditions in the water bath or
Czech name
—
Czech description
—

Project
<a href="/en/project/VF20112015013" target="_blank" >VF20112015013: Research of modern methods of detection and identification of hazardous CBRN substances and materials, methods of hazard reduction and decontamination; research of modern method for personal protection and elements of critical infrastructure</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
26. KONGRES ČESKOSLOVENSKÉ SPOLEČNOSTI MIKROBIOLOGICKÉ
ISBN
978-80-260-4507-6
ISSN
—
e-ISSN
—
Number of pages
1
Pages from-to
163
Publisher name
Československá společnost mikrobiologická
Place of publication
Praha-Bratislava
Event location
Brno
Event date
Jan 1, 2013
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
—

Similar results(10)