Insect silk contains both a Kunitz-type and a unique Kazal-type proteinase inhibitor

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F01%3A00003155" target="_blank" >RIV/60076658:12310/01:00003155 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Insect silk contains both a Kunitz-type and a unique Kazal-type proteinase inhibitor

Original language description

In addition to the fibrous (fibroins) and sticky (sericins) components, the silk contains several small peptides of unknown function. We demonstrate that two of the small peptides present in the silk of the waxmoth, Galleria mellonella, inhibit proteolytic enzymes, and are thus called silk proteinase inhibitors 1 and 2 (SPI 1, SPI 2). They are produced in the middle section of the silk-secreting glands prior to cocoon spinning and their production is controlled at the transcription level. The full length cDNA of pre-SPI 1 contains 443 nt and encodes a peptide of 76 amino acid residues, of which 20 make up a signal sequence. The mature SPI 1 (6056.7 Da, 56 residues) is a typical thermostable Kunitz-type proteinase inhibitor with Arg in P1 position. ThecDNA of pre-SPI 2 consists of 260 nt and yields a putative secretory peptide of 58 amino acid residues. The functional SPI 2 (3993 Da, 36 residues), generated after the signal sequence removal, is a single-domain Kazal-type proteinase inh

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FB - Endocrinology, diabetology, metabolism, nutrition

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2001

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

European Journal of Biochemistry

ISSN

0014-2956

e-ISSN

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Volume of the periodical

268

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

2064-2073

UT code for WoS article

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EID of the result in the Scopus database

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