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ČeštinaEnglish

LC/MS analysis of adipokinetic peptides in the Pyrrhocoris apterus (Heteroptera)

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F01%3A00003161" target="_blank" >RIV/60076658:12310/01:00003161 - isvavai.cz</a>

  • Result on the web

    —

  • DOI - Digital Object Identifier

    —

Alternative languages

  • Result language

    angličtina

  • Original language name

    LC/MS analysis of adipokinetic peptides in the Pyrrhocoris apterus (Heteroptera)

  • Original language description

    An LC/ESI/MS/MS method was developed for quantitative analysis of peptides from the adipokinetic hormone/red pigment concentrating hormone family (AKH/RPCH family) in insects. The peptides were extracted by aqueous methanol, the extract defated and the aqueous layer analysed by LC/ESI/MSN. With the developed methodology low femtomole levels can be detected allowing thus direct AKH sequencing and analysis in the corpora cardiaca from one individual insect. Direct examination of corpora cardiaca of the P.apterus revealed a new adipokinetic peptide. It is an octapeptide designated as Pya-AKH-II with the sequence of pGlu-Leu-Thr-Phe-Thr-Pro-Asn-Trp-NH2. The new peptide is active in the lipid mobilisation assay, but its activity seems to be lower than withthe Pya-AKH-I. The identity of the Pya-AKH-II was definitively confirmed by synthesis.

  • Czech name

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  • Czech description

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Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    ED - Physiology

  • OECD FORD branch

    —

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2001

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Collection Symposium Series

  • ISSN

    xxxx-xxxx

  • e-ISSN

    —

  • Volume of the periodical

    4

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    3

  • Pages from-to

    90-92

  • UT code for WoS article

    —

  • EID of the result in the Scopus database

    —

Similar results(10)

LC/MS analysis of adipokinetic peptides in the Pyrrhocoris apterus (Heteroptera).Identification and bioactivity evaluation of the first neuropeptide from the lesser-known insect order Embioptera (webspinner)Pya-AKH-II- a new peptide from the AKH/RPCH family was identified in the corpora cardiaca of the firebug, Pyrrhocoris apterus (Heteroptera)