Wild chimpanzees are infected by Trypanosoma brucei

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F15%3A43889147" target="_blank" >RIV/60076658:12310/15:43889147 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/15:00453377 RIV/00216208:11310/15:10321917 RIV/62157124:16170/15:43873366 RIV/62157124:16810/15:43873366

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S2213224415000243" target="_blank" >http://www.sciencedirect.com/science/article/pii/S2213224415000243</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijppaw.2015.05.001" target="_blank" >10.1016/j.ijppaw.2015.05.001</a>

Result language

angličtina

Original language name

Wild chimpanzees are infected by Trypanosoma brucei

Original language description

sickness, very little is known about their trypanosome infections, mainly due to major difficulties in obtaining their blood samples. In present work, we established a diagnostic ITS1-based PCR assay that allows detection of the DNA of all four Trypanosoma brucei subspecies (Trypanosoma brucei brucei, Trypanosoma brucei rhodesiense, Trypanosoma brucei gambiense, and Trypanosoma brucei evansi) in feces of experimentally infected mice. Next, using this assay we revealed the presence of trypanosomes in the fecal samples of wild chimpanzees and this finding was further supported by results obtained using a set of primate tissue samples. Phylogenetic analysis of the ITS1 region showed that the majority of obtained sequences fell into the robust T. brucei group, providing strong evidence that these infections were caused by T. b. rhodesiense and/or T. b. gambiense. The optimized technique of trypanosome detection in feces will improve our knowledge about the epidemiology of trypanosomes in primates and possibly also other endangered mammals, from which blood and tissue samples cannot be obtained. Finally, we demonstrated that the mandrill serum was able to efficiently lyse T. b. brucei and T. b. rhodesiense, and to some extent T. b. gambiense, while the chimpanzee serum failed to lyse any of these subspecies.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal for Parasitology: Parasites and Wildlife

ISSN

2213-2244

e-ISSN

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Volume of the periodical

4

Issue of the periodical within the volume

2015

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

277-282

UT code for WoS article

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EID of the result in the Scopus database

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