Wild chimpanzees are infected by Trypanosoma brucei
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F15%3A00445138" target="_blank" >RIV/68081766:_____/15:00445138 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.ijppaw.2015.05.001" target="_blank" >http://dx.doi.org/10.1016/j.ijppaw.2015.05.001</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ijppaw.2015.05.001" target="_blank" >10.1016/j.ijppaw.2015.05.001</a>
Alternative languages
Result language
angličtina
Original language name
Wild chimpanzees are infected by Trypanosoma brucei
Original language description
Although wild chimpanzees and other African great apes live in regions endemic for African sleeping sickness, very little is known about their trypanosome infections, mainly due to major difficulties in obtaining their blood samples. In present work, we established a diagnostic ITS1-based PCR assay that allows detection of the DNA of all four Trypanosoma brucei subspecies (Trypanosoma brucei brucei, Trypanosoma brucei rhodesiense, Trypanosoma brucei gambiense, and Trypanosoma brucei evansi) in feces of experimentally infected mice. Next, using this assay we revealed the presence of trypanosomes in the fecal samples of wild chimpanzees and this finding was further supported by results obtained using a set of primate tissue samples. Phylogenetic analysis of the ITS1 region showed that the majority of obtained sequences fell into the robust T. brucei group, providing strong evidence that these infections were caused by T. b. rhodesiense and/or T. b. gambiense. The optimized technique of trypanosome detection in feces will improve our knowledge about the epidemiology of trypanosomes in primates and possibly also other endangered mammals, from which blood and tissue samples cannot be obtained. Finally, we demonstrated that the mandrill serum was able to efficiently lyse T. b. brucei and T. b. rhodesiense, and to some extent T. b. gambiense, while the chimpanzee serum failed to lyse any of these subspecies.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10618 - Ecology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal for Parasitology
ISSN
0020-7519
e-ISSN
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Volume of the periodical
4
Issue of the periodical within the volume
3
Country of publishing house
AU - AUSTRALIA
Number of pages
6
Pages from-to
277-282
UT code for WoS article
000371441100001
EID of the result in the Scopus database
2-s2.0-84944442238