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Wild chimpanzees are infected by Trypanosoma brucei

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F15%3A00445138" target="_blank" >RIV/68081766:_____/15:00445138 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1016/j.ijppaw.2015.05.001" target="_blank" >http://dx.doi.org/10.1016/j.ijppaw.2015.05.001</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.ijppaw.2015.05.001" target="_blank" >10.1016/j.ijppaw.2015.05.001</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Wild chimpanzees are infected by Trypanosoma brucei

  • Original language description

    Although wild chimpanzees and other African great apes live in regions endemic for African sleeping sickness, very little is known about their trypanosome infections, mainly due to major difficulties in obtaining their blood samples. In present work, we established a diagnostic ITS1-based PCR assay that allows detection of the DNA of all four Trypanosoma brucei subspecies (Trypanosoma brucei brucei, Trypanosoma brucei rhodesiense, Trypanosoma brucei gambiense, and Trypanosoma brucei evansi) in feces of experimentally infected mice. Next, using this assay we revealed the presence of trypanosomes in the fecal samples of wild chimpanzees and this finding was further supported by results obtained using a set of primate tissue samples. Phylogenetic analysis of the ITS1 region showed that the majority of obtained sequences fell into the robust T. brucei group, providing strong evidence that these infections were caused by T. b. rhodesiense and/or T. b. gambiense. The optimized technique of trypanosome detection in feces will improve our knowledge about the epidemiology of trypanosomes in primates and possibly also other endangered mammals, from which blood and tissue samples cannot be obtained. Finally, we demonstrated that the mandrill serum was able to efficiently lyse T. b. brucei and T. b. rhodesiense, and to some extent T. b. gambiense, while the chimpanzee serum failed to lyse any of these subspecies.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10618 - Ecology

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    International Journal for Parasitology

  • ISSN

    0020-7519

  • e-ISSN

  • Volume of the periodical

    4

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    AU - AUSTRALIA

  • Number of pages

    6

  • Pages from-to

    277-282

  • UT code for WoS article

    000371441100001

  • EID of the result in the Scopus database

    2-s2.0-84944442238