Cryo-imaging of photosystems and phycobilisomes in Anabaena sp PCC 7120 cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F15%3A43890194" target="_blank" >RIV/60076658:12310/15:43890194 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/15:00454951
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S1011134415003243" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1011134415003243</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jphotobiol.2015.10.003" target="_blank" >10.1016/j.jphotobiol.2015.10.003</a>

Result language
angličtina
Original language name
Cryo-imaging of photosystems and phycobilisomes in Anabaena sp PCC 7120 cells
Original language description
Primary photosynthetic reactions take place inside thylakoid membrane where light-to-chemical energy conversion is catalyzed by two pigment-protein complexes, photosystem I (PSI) and photosystem II (PSII). Light absorption in cyanobacteria is increased by pigment-protein supercomplexes phycobilisomes (PBSs) situated on thylakoid membrane surfaces that transfer excitation energy into both photosystems. We have explored the localization of PSI, PSII and PBSs in thylakoid membrane of native cyanobacteria cell Anabaena sp. 7120 by means of cryogenic con-focal microscopy. We have adapted a conventional temperature controlling stage to an Olympus FV1000 confocal microscope. The presence of red shifted emission of chlorophylls from PSI has been confirmed by spectral measurements. Confocal fluorescence images of PSI (in a spectral range 710-750 nm), PSII (in a spectral range 690-705 nm) and PBSs (in a spectral range 650-680 nm) were recorded at low temperature. Co-localization of images showed
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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