Protein arrangement factor: a new photosynthetic parameter characterizing the organization of thylakoid membrane proteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00505346" target="_blank" >RIV/61388971:_____/19:00505346 - isvavai.cz</a>

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.12952" target="_blank" >https://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.12952</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/ppl.12952" target="_blank" >10.1111/ppl.12952</a>

Result language

angličtina

Original language name

Protein arrangement factor: a new photosynthetic parameter characterizing the organization of thylakoid membrane proteins

Original language description

A proper spatial distribution of photosynthetic pigment-protein complexes-PPCs (photosystems, light-harvesting antennas) is crucial for photosynthesis. In plants, photosystems I and II (PSI and PSII) are heterogeneously distributed between granal and stromal thylakoids. Here we have described similar heterogeneity in the PSI, PSII and phycobilisomes (PBSs) distribution in cyanobacteria thylakoids into microdomains by applying a new image processing method suitable for the Synechocystis sp. PCC6803 strain with yellow fluorescent protein-tagged PSI. The new image processing method is able to analyze the fluorescence ratios of PPCs on a single-cell level, pixel per pixel. Each cell pixel is plotted in CIE1931 color space by forming a pixel-color distribution of the cell. The most common position in CIE1931 is then defined as protein arrangement (PA) factor with xy coordinates. The PA-factor represents the most abundant fluorescence ratio of PSI/PSII/PBS, the mode color' of studied cell. We proved that a shift of the PA-factor from the center of the cell-pixel distribution (the median' cell color) is an indicator of the presence of special subcellular microdomain(s) with a unique PSI/PSII/PBS fluorescence ratio in comparison to other parts of the cell. Furthermore, during a 6-h high-light (HL) treatment, median' and mode' color (PA-factor) of the cell changed similarly on the population level, indicating that such microdomains with unique PSI/PSII/PBS fluorescence were not formed during HL (i.e. fluorescence changed equally in the whole cell). However, the PA-factor was very sensitive in characterizing the fluorescence ratios of PSI/PSII/PBS in cyanobacterial cells during HL by depicting a 4-phase acclimation to HL, and their physiological interpretation has been discussed.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10611 - Plant sciences, botany

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Physiologia Plantarum

ISSN

0031-9317

e-ISSN

—

Volume of the periodical

166

Issue of the periodical within the volume

1 SI

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

264-277

UT code for WoS article

000466108300021

EID of the result in the Scopus database

2-s2.0-85064648839