A calcium-accumulating region, CAR, in the channel Orai1 enhances Ca2+ permeation and SOCE-induced gene transcription

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F15%3A43890218" target="_blank" >RIV/60076658:12310/15:43890218 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/15:00463207

Result on the web

<a href="http://stke.sciencemag.org/content/8/408/ra131" target="_blank" >http://stke.sciencemag.org/content/8/408/ra131</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1126/scisignal.aab1901" target="_blank" >10.1126/scisignal.aab1901</a>

Result language

angličtina

Original language name

A calcium-accumulating region, CAR, in the channel Orai1 enhances Ca2+ permeation and SOCE-induced gene transcription

Original language description

The Ca2+ release-activated Ca2+ channel mediates Ca2+ influx in a plethora of cell types, thereby controlling diverse cellular functions. The channel complex is composed of stromal interaction molecule 1 (STIM1), an endoplasmic reticulum Ca2+-sensing protein, and Orai1, a plasma membrane Ca2+ channel. Channels composed of STIM1 and Orai1 mediate Ca2+ influx even at low extracellular Ca2+ concentrations. We investigated whether the activity of Orai1 adapted to different environmental Ca2+ concentrations.We used homology modeling and molecular dynamics simulations to predict the presence of an extracellular Ca2+-accumulating region (CAR) at the pore entrance of Orai1. Furthermore, simulations of Orai1 proteins with mutations in CAR, along with live-cellexperiments, or simulations and electrophysiological recordings of the channel with transient, electrostatic loop3 interacting with loop1 (the site of CAR) determined that CAR enhanced Ca2+ permeation most efficiently at low external Ca2

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

<a href="/en/project/GA13-21053S" target="_blank" >GA13-21053S: Investigating the Symmetric Allosteric Mechanism of Hexameric Escherichia coli Arginine Repressor using Computational Simulations</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Science Signaling

ISSN

1945-0877

e-ISSN

—

Volume of the periodical

8

Issue of the periodical within the volume

408

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

—

UT code for WoS article

000367102600004

EID of the result in the Scopus database

—