Neovahlkampfia nana n. sp Reinforcing an Underrepresented Subclade of Tetramitia, Heterolobosea

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F17%3A43895478" target="_blank" >RIV/60076658:12310/17:43895478 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/17:00094556
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1111/jeu.12341/abstract;jsessionid=A9D8323BC59677CE806E681153952972.f02t02" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/jeu.12341/abstract;jsessionid=A9D8323BC59677CE806E681153952972.f02t02</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jeu.12341" target="_blank" >10.1111/jeu.12341</a>

Result language
angličtina
Original language name
Neovahlkampfia nana n. sp Reinforcing an Underrepresented Subclade of Tetramitia, Heterolobosea
Original language description
The study provides robust genetic evidence that a newly isolated naked ameba with morphological and ultrastructural features indicative of Heterolobosea is a new species. Neovahlkampfia nana n. sp. associates with the yet underrepresented subclade of Tetramitia I. Considerable differences found in 18S rRNA gene sequences of individual molecular clones derived from DNA of five clonal cultures, using a low fidelity DNA polymerase, raised the issue of intragenomic sequence variation, a phenomenon that has not been previously studied in Heterolobosea. However, as proved using a higher fidelity DNA polymerase, the sequence variability observed was introduced by PCR mediated by the low fidelity polymerase and fixed by molecular cloning. This points to the potentially dubious validity of some current nominal species of Heterolobosea that differ from one another in just one or two base positions.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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