Analysis of nucleotide sequence of Pisum sativum chromosome-5-specific library

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F04%3A00109137" target="_blank" >RIV/61389030:_____/04:00109137 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Analysis of nucleotide sequence of Pisum sativum chromosome-5-specific library
Original language description
Sequencing of clones derived from short insert chromosome-5-specific DNA library of pea may provide valuable information of chromosome structure. Our library was constructed by degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) amplification using DNA of flow-sorted chromosomes as template. Analysis of nucleotide sequence of 200 randomly selected clones indicated that about 20% of clones were overrepresented in the library. The nucleotide sequence of 155 original chromosome-specific fragments was revealed. Sequence homology analysis showed that minority of fragments were similar only to uncharacterised ge-nomic sequences of different plant species. However, the set of sequences revealed different ranges of similarity to plant genes and different types of moderate repetitive elements. The results suggested equal reflection of real genome composition in the library clones, with the exception of short tandem repeats sequences. Shotgun characterisation implied a rathe...
Czech name
Analýza sekvencí chromozomu 5 Pisum sativum
Czech description
Sekvenování klonů z knihovny specifické pro chromozom 5 může poskytnout hodnotné informace o jeho struktuře. Knihovna byla konstruována z tříděných chromozomů amplifikovaných pomcí DOP PCR. Bylo sekvenováno a analyzováno a 200 klonů této knihovny.

Project
<a href="/en/project/GA521%2F03%2F0595" target="_blank" >GA521/03/0595: Targeted isolation of molecular markers and comparative genome mapping in chickpea, field bean and garden pea</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Proceedings of the International Workshop 'Understanding the Plant Genomeö
ISBN
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ISSN
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e-ISSN
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Number of pages
10
Pages from-to
79-88
Publisher name
Institute of Plant Genetics PAS
Place of publication
Poznan
Event location
Poznan
Event date
Jun 2, 2003
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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