Impact of cryopreservation on sterlet, Acipenser ruthenus sperm motility and proteome

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897122" target="_blank" >RIV/60076658:12310/18:43897122 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/18:00498849 RIV/60076658:12520/18:43897122

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0378432018301702" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0378432018301702</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.anireprosci.2018.03.025" target="_blank" >10.1016/j.anireprosci.2018.03.025</a>

Result language

angličtina

Original language name

Impact of cryopreservation on sterlet, Acipenser ruthenus sperm motility and proteome

Original language description

Fish sperm cryopreservation is a well-established technique allowing for artificial insemination on a commercial scale. The extent of proteome alterations in seminal plasma and sperm due to cryopreservation, however, is not known. This study was conducted to evaluate the effect of cryopreservation on motility variables of sterlet Acipenser ruthenus sperm and to detect the differences in protein profiles of fresh and cryopreserved sterlet sperm and seminal plasma. Fresh sperm had 89 +/- 3% motility and 160 +/- 14 mu m/s curvilinear velocity at 15 s post-activation. The motility rate of cryopreserved sperm (37 +/- 5%) was less at 15 s post-activation. No difference (ANOVA; P &gt; 0.05) in mean curvilinear velocity of fresh and cryopreserved sperm was detected. The protein profiles of seminal plasma and sperm were characterized using comparative proteomics to determine the influence of cryopreservation. Six altered protein spots in seminal plasma and thirteen altered spots in sperm were detected in fresh and thawed sperm. Subsequent protein characterization suggested that the proteins identified were involved in sperm metabolism, cytoskeleton, and stress response. The results broaden the understanding of the effects of cryopreservation and identify the proteins associated with cryo-injury. These data may help to determine the function of altered proteins and provide new insights into improving sperm cryopreservation.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40103 - Fishery

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Animal Reproduction Science

ISSN

0378-4320

e-ISSN

—

Volume of the periodical

192

Issue of the periodical within the volume

05/2018

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

10

Pages from-to

280-289

UT code for WoS article

000431163900034

EID of the result in the Scopus database

2-s2.0-85044531629