Adipokinetic hormone and adenosine interfere with nematobacterial infection and locomotion in Drosophila melanogaster
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897407" target="_blank" >RIV/60076658:12310/18:43897407 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/18:00489356 RIV/00216224:14310/18:00100913
Result on the web
<a href="https://reader.elsevier.com/reader/sd/pii/S0022191018300787?token=DE6D13E92A357D73805EF2EC4279838625701F67266BF7AB661E1494D2CA6C9A3A26DCC33E890E364012B34B3346FC90" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0022191018300787?token=DE6D13E92A357D73805EF2EC4279838625701F67266BF7AB661E1494D2CA6C9A3A26DCC33E890E364012B34B3346FC90</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jinsphys.2018.04.002" target="_blank" >10.1016/j.jinsphys.2018.04.002</a>
Alternative languages
Result language
angličtina
Original language name
Adipokinetic hormone and adenosine interfere with nematobacterial infection and locomotion in Drosophila melanogaster
Original language description
This study examined how adipokinetic hormone (AKH) and adenosine affect defense responses in Drosophila melanogaster larvae infected with entomopathogenic nematodes (EPN, Steinernema carpocapsae and Heterorhabditis bacteriophora). Three loss-of-function mutant larvae were tested: Akh(1), AdoR(1) (adenosine receptor), and Akh(1) AdoR(1). Mortality decreased in all mutants post-EPN infection compared with the control (w(1118)). Additionally, co-application of external AKH with EPN significantly increased mortality beyond rates observed in EPN-only treatment, while also elevating carbon dioxide production, a measure of metabolism. Furthermore trehalose levels increased in both w(1118) and Akh(1) larvae post-EPN infection, but the latter group exhibited a lower increase and total trehalose levels. Interestingly, baseline trehalose was relatively high in untreated AdoR(1) and Akh(1) AdoR(1) mutants, with levels remaining unaffected by infection. Infection also elevated haemolymph lipid content overall, but the different mutations did not substantially influence this change. In contrast, haemolymph protein content dropped after EPN infection in all tested groups, but this decline was more intense among Akh(1). In uninfected larvae mutations decreased antioxidative capacity in Akh(1) and increased in AdoR(1), however, its post-infection increases were similar in all mutants, suggesting that antioxidant response in Drosophila involves mechanisms also beyond AKH and adenosine. Furthermore, AKH application in w(1118) larvae significantly increased movement distance and percentage of larval activity, but reduced velocity. Mutations of Akh and AdoR did not strongly affect locomotion.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA17-03253S" target="_blank" >GA17-03253S: Hormonal control of insect defence system</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Insect Physiology
ISSN
0022-1910
e-ISSN
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Volume of the periodical
107
Issue of the periodical within the volume
MAY-JUN 2018
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
167-174
UT code for WoS article
000434751100021
EID of the result in the Scopus database
2-s2.0-85045386083