Multiple legumain legumain isoenzymes in ticks

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897425" target="_blank" >RIV/60076658:12310/18:43897425 - isvavai.cz</a>

Result on the web

<a href="https://reader.elsevier.com/reader/sd/pii/S0020751917303016?token=F9D75D5A653977B46743E43E8ADC8BD3E40A09CC2B3FF3F09D09CBCC804734EE645C075AA9553EE0DA9105BBBE661692" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0020751917303016?token=F9D75D5A653977B46743E43E8ADC8BD3E40A09CC2B3FF3F09D09CBCC804734EE645C075AA9553EE0DA9105BBBE661692</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijpara.2017.08.011" target="_blank" >10.1016/j.ijpara.2017.08.011</a>

Result language

angličtina

Original language name

Multiple legumain legumain isoenzymes in ticks

Original language description

By searching nucleotide databases for the North American Lyme disease vector, Ixodes scapularis, we have complemented the previously characterized European Ixodes ricinus legumain IrAE1 with a full set of nine analogous genes (isael-9). Six of these were PCR confirmed as genes present in all tick genomes tested. The absolute mRNA copy number examined by quantitative (q)PCR enabled expression profiling and an absolute comparison of mRNA levels for individual I. scapularis (Is)AEs in tick tissues. Four IsAEs (1, 2, 4, 9) were expressed solely in the gut and thus are proposed to be involved in host blood digestion. Expression qPCR profiling over developmental stages confirmed IsAE1, the direct analogue of previously characterized I. ricinus IrAE1, as the principle legumain transcript in partially engorged females, and demonstrated its strong regulation by on-host feeding in larvae, nymphs and females. In contrast, IsAE2 was the predominant gut legumain in unfed nymphs, unfed females and males. In-silico, IsAE1 and IsAE2 protein three-dimensional structural models displayed minimal differences in overall proenzyme structures, even in comparison with recently resolved crystal structures of mammalian prolegumain. Three functional studies were performed in I. ricinus with IsAE1/IsAE2 analogues: double IrAE1/lrAE2 RNA interference silencing, feeding of ticks on IrAEl+IrAE2 immunized hosts and in vitro membrane tick feeding on blood containing a legumain-specific inhibitor. The latter experiment led to reduced weights of fully engorged ticks and limited oviposition, and indicated the potential of legumain inhibitors for novel anti-tick interventions.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal for Parasitology

ISSN

0020-7519

e-ISSN

—

Volume of the periodical

48

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

167-178

UT code for WoS article

000425070300008

EID of the result in the Scopus database

2-s2.0-85034450749