Ploidy and Number of Chromosomes in the Alveolate Alga Chromera velia
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897587" target="_blank" >RIV/60076658:12310/18:43897587 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/18:00498839 RIV/61388971:_____/18:00490079
Result on the web
<a href="https://reader.elsevier.com/reader/sd/pii/S1434461017301062?token=19918AD415736D2BF6F3BFBB7E141AB6D4F002A09BCE6CD4DBA47FAAA2E749C944FFCBAA11FBB8B69A5EE05237068F8D" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S1434461017301062?token=19918AD415736D2BF6F3BFBB7E141AB6D4F002A09BCE6CD4DBA47FAAA2E749C944FFCBAA11FBB8B69A5EE05237068F8D</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.protis.2017.12.001" target="_blank" >10.1016/j.protis.2017.12.001</a>
Alternative languages
Result language
angličtina
Original language name
Ploidy and Number of Chromosomes in the Alveolate Alga Chromera velia
Original language description
Chromera velia is an alveolate alga which represents the closest known phototrophic relative to apicomplexan parasites. Although the nuclear, mitochondrial, and plastid genomes of this alga have been sequenced, the number of chromosomes and ploidy of C. velia are unknown. We explored ploidy in the vegetative cell, the predominant stage in cultures of Chromera, using the tyramide signal amplification-fluorescence in situ hybridization (TSA-FISH) in isolated nuclei of C. velia. Probes were derived from three single copy genes coding for 4-diphosphocytidyl-2-C-methyl-D-erythritol (CDP-ME) kinase, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MEcPP) synthase and Topoisomerase II. Our results indicate that the vegetative cell of C. velia is haploid, as each probe produced a single fluorescent signal, although the possibility of diploidy with somatic pairing of homologous chromosomes cannot be completely excluded. Restriction analysis and hybridization with the telomere probe produced eight bands suggesting the presence of four chromosomes in haploid vegetative cells of C. velia. However, when the chromerid-specific telomere probe (TTTAGGG)(4) was used for TSA-FISH, we consistently obtained a double signal. This may indicate that the four chromosomes are organized in clusters in interphase nuclei of C. velia, which is a chromosome organization similar to that of their apicomplexan relatives. (C) 2017 Elsevier GmbH. All rights reserved.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Protist
ISSN
1434-4610
e-ISSN
—
Volume of the periodical
169
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
53-63
UT code for WoS article
000427418800005
EID of the result in the Scopus database
2-s2.0-85041563662