Investigation of the genome sizes and ploidy within the genus Monocercomonoides
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10449635" target="_blank" >RIV/00216208:11310/22:10449635 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=CqshtP3yuN" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=CqshtP3yuN</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jeu.12925" target="_blank" >10.1111/jeu.12925</a>
Alternative languages
Result language
angličtina
Original language name
Investigation of the genome sizes and ploidy within the genus Monocercomonoides
Original language description
Monocercomonoides is a genus of anaerobic flagellates found mainly in the gut of insects and vertebrates. We explored the ploidy of six strains of Monocercomonoides using fluorescence in situ hybridization (FISH) with probes against the SufDSU gene known to be in a single copy in M. exilis. Our results show that all investigated strains are haploid, with a single clear signal displayed in most of the analyzed nuclei. Staining of the telomeric repeats TTAGGG using FISH revealed that all investigated strains, except for strains of M. merkovicensis, exhibit a similar number of telomeric signals to those of M. exilis. DNA content of the nuclei in seven strains was assessed using flow cytometry. With the knowledge of the ploidy, their haploid genome sizes were estimated to vary from 60 to 161 Mbp. The genome size variation observed in Monocercomonoides is much larger than the variation within other genera of metamonads such as Trichomonas, Tritrichomonas, or Giardia, but similar to the variations observed within genera of algae or plants.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Eukaryotic Microbiology
ISSN
1066-5234
e-ISSN
1550-7408
Volume of the periodical
69
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
nestrankovano
UT code for WoS article
000807228600001
EID of the result in the Scopus database
2-s2.0-85131228275