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Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897648" target="_blank" >RIV/60076658:12310/18:43897648 - isvavai.cz</a>

  • Alternative codes found

    RIV/60077344:_____/18:00490983

  • Result on the web

    <a href="https://www.jove.com/video/57385/practical-use-rna-interference-oral-delivery-double-stranded-rna" target="_blank" >https://www.jove.com/video/57385/practical-use-rna-interference-oral-delivery-double-stranded-rna</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3791/57385" target="_blank" >10.3791/57385</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches

  • Original language description

    RNA interference (RNAi) has been widely applied for uncovering the biological functions of numerous genes, and has been envisaged as a pest control tool operating by disruption of essential gene expression. Although different methods, such as injection, feeding, and soaking, have been reported for successful delivery of double-stranded RNA (dsRNA), the efficiency of RNAi through oral delivery of dsRNA is highly variable among different insect groups. The German cockroach, Blattella germanica, is highly sensitive to the injection of dsRNA, as shown by many studies published previously. The present study describes a method to demonstrate that the dsRNA encapsulated with liposome carriers is sufficient to retard the degradation of dsRNA by midgut juice. Notably, the continuous feeding of dsRNA encapsulated by liposomes significantly reduces the tubulin expression in the midgut, and led to the death of cockroaches. In conclusion, the formulation and utilization of dsRNA lipoplexes, which protect dsRNA against nucleases, could be a practical use of RNAi for insect pest control in the future.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    JoVE - Journal of Visualized Experiments

  • ISSN

    1940-087X

  • e-ISSN

  • Volume of the periodical

    neuveden

  • Issue of the periodical within the volume

    135

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    6

  • Pages from-to

  • UT code for WoS article

    000444372200068

  • EID of the result in the Scopus database

    2-s2.0-85046680407