Method for Extraction and Quantification of Metal-Based Nanoparticles in Biological Media: Number-Based Biodistribution and Bioconcentration

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F19%3A43899075" target="_blank" >RIV/60076658:12310/19:43899075 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12520/19:43899075

Result on the web

<a href="https://pubs.acs.org/doi/full/10.1021/acs.est.8b03715" target="_blank" >https://pubs.acs.org/doi/full/10.1021/acs.est.8b03715</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.est.8b03715" target="_blank" >10.1021/acs.est.8b03715</a>

Result language

angličtina

Original language name

Method for Extraction and Quantification of Metal-Based Nanoparticles in Biological Media: Number-Based Biodistribution and Bioconcentration

Original language description

A multistep sample preparation method was developed to separate metal-based engineered nanoparticles (ENPs) from biological samples. The method was developed using spiked zebrafish tissues and standard titanium dioxide (TiO2) and cerium dioxide (CeO2) ENPs. Single-particle inductively coupled plasma mass spectrometry was used to quantify the separated particles in terms of number concentration. This method demonstrated mass recoveries of more than 90% and did not strikingly alter the median particles size. High number recoveries were calculated for CeO2 ENPs (&gt;84%). Particle number recoveries were poor for TiO2 ENPs (&lt;25%), which could be due to the interference of Ca-48 with the measured isotope Ti-48. The method was verified using zebrafish exposed to CeO2 ENPs to test its applicability for nanotoxicokinetic investigations. Total mass of Ce and particle number concentration of CeO2 ENPs were measured in different tissues. Notably, the mass-based biodistribution of Ce in the tissues did not follow the number-based biodistribution of CeO2. Moreover, the calculated mass-based bioconcentration factors showed a different pattern in comparison to the number-based bioconcentration factors. Our findings suggest that considering mass as the sole dose-metric may not provide sufficient information to investigate toxicity and toxicokinetics of ENPs.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Environmental Science &amp; Technology

ISSN

0013-936X

e-ISSN

—

Volume of the periodical

53

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

946-953

UT code for WoS article

000456349900042

EID of the result in the Scopus database

2-s2.0-85059930943