Structural and spectroscopic characterization of HCP2

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F19%3A43899146" target="_blank" >RIV/60076658:12310/19:43899146 - isvavai.cz</a>
Result on the web
<a href="https://reader.elsevier.com/reader/sd/pii/S0005272819300192?token=73B9353559540D388823196A91DD9BADA77CFF5D46313CE4EAD5A25BC8651AC8F5DD16F7B4BB7C8388811113202CA533" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0005272819300192?token=73B9353559540D388823196A91DD9BADA77CFF5D46313CE4EAD5A25BC8651AC8F5DD16F7B4BB7C8388811113202CA533</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bbabio.2019.03.004" target="_blank" >10.1016/j.bbabio.2019.03.004</a>

Result language
angličtina
Original language name
Structural and spectroscopic characterization of HCP2
Original language description
The Helical Carotenoid Proteins (HCPs) are a large group of newly identified carotenoid-binding proteins found in ecophysiologically diverse cyanobacteria. They likely evolved before becoming the effector (quenching) domain of the modular Orange Carotenoid Protein (OCP). The number of discrete HCP families-at least nine-suggests they are involved in multiple distinct functions. Here we report the 1.7 angstrom crystal structure of HCP2, one of the most widespread HCPs found in nature, from the chromatically acclimating cyanobacterium Tolypothrix sp. PCC 7601. By purifying HCP2 from the native source we are able to identify its natively-bound carotenoid, which is exclusively canthaxanthin. In solution, HCP2 is a monomer with an absorbance maximum of 530 nm. However, the HCP2 crystals have a maximum absorbance at 548 nm, which is accounted by the stacking of the beta 1 rings of the carotenoid in the two molecules in the asymmetric unit. Our results demonstrate how HCPs provide a valuable system to study carotenoid-protein interactions and their spectroscopic implications, and contribute to efforts to understand the functional roles of this large, newly discovered family of pigment proteins, which to-date remain enigmatic.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology

Project
<a href="/en/project/GA18-21631S" target="_blank" >GA18-21631S: Ultrafast spectroscopy as a tool for elucidation the structure-function relationship in cyanobacterial carotenoid-binding proteins</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)