Regulation of macrophage activity by surface receptors contained within Borrelia burgdorferi-enriched phagosomal fractions

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F19%3A43899864" target="_blank" >RIV/60076658:12310/19:43899864 - isvavai.cz</a>

Alternative codes found

RIV/27283933:_____/19:00007352

Result on the web

<a href="https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1008163&type=printable" target="_blank" >https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1008163&type=printable</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.ppat.1008163" target="_blank" >10.1371/journal.ppat.1008163</a>

Result language

angličtina

Original language name

Regulation of macrophage activity by surface receptors contained within Borrelia burgdorferi-enriched phagosomal fractions

Original language description

Macrophages mediate the elimination of pathogens by phagocytosis resulting in the activation of specific signaling pathways that lead to the production of cytokines, chemokines and other factors. Borrelia burgdorferi, the causative agent of Lyme disease, causes a wide variety of pro-inflammatory symptoms. The proinflammatory capacity of macrophages is intimately related to the internalization of the spirochete. However, most receptors mediating this process are largely unknown. We have applied a multiomic approach, including the proteomic analysis of B. burgdorferi-containing phagosome-enriched fractions, to identify surface receptors that are involved in the phagocytic capacity of macrophages as well as their inflammatory output. Sucrose gradient protein fractions of human monocyte-derived macrophages exposed to B. burgdorferi contained the phagocytic receptor, CR3/CD14 highlighting the major role played by these proteins in spirochetal phagocytosis. Other proteins identified in these fractions include C-type lectins, scavenger receptors or Siglecs, of which some are directly involved in the interaction with the spirochete. We also identified the Fc gamma receptor pathway, including the binding receptor, CD64, as involved both in the phagocytosis of, and TNF induction in response to B. burgdorferi in the absence of antibodies. The common gamma chain, Fc gamma R, mediates the phagocytosis of the spirochete, likely through Fc receptors and C-type lectins, in a process that involves Syk activation. Overall, these findings highlight the complex array of receptors involved in the phagocytic response of macrophages to B. burgdorferi.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS Pathogens

ISSN

1553-7366

e-ISSN

—

Volume of the periodical

15

Issue of the periodical within the volume

11

Country of publishing house

US - UNITED STATES

Number of pages

23

Pages from-to

—

UT code for WoS article

000503029900034

EID of the result in the Scopus database

—