Molecular and subcellular cryoinjury of fish spermatozoa and approaches to improve cryopreservation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43900880" target="_blank" >RIV/60076658:12310/20:43900880 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/20:00541307 RIV/60076658:12520/20:43900880
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/full/10.1111/raq.12355" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1111/raq.12355</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/raq.12355" target="_blank" >10.1111/raq.12355</a>
Alternative languages
Result language
angličtina
Original language name
Molecular and subcellular cryoinjury of fish spermatozoa and approaches to improve cryopreservation
Original language description
The quality of frozen/thawed fish sperm is generally lower than that of fresh sperm. Extremely low temperatures are associated with damage to fish spermatozoon subcellular compartments and associated molecules such as DNA and proteins. Cryodamage can negatively affect DNA integrity, spermatozoon metabolism and motility, consequently impairing fertilization and embryo development. To preserve sperm efficiently, the addition of proteins including seminal plasma proteins, antioxidants, antifreeze proteins and antifreeze glycoproteins, and bovine serum albumin before and/or after freezing is suggested to minimize cryoinjury. An appropriate quantity of seminal plasma components in cryopreservation medium can prevent spermatozoon membrane damage by maintaining antioxidant enzymes and their proper distribution on the spermatozoon surface. Under storage conditions, the semen antioxidant system is ineffective in protecting spermatozoa from reactive oxygen species. The addition of antioxidants can provide protection for spermatozoa and reduce cryoinjuries during storage, but the effect of a given antioxidant is species-specific. Antifreeze proteins and antifreeze glycoproteins can stabilize the cell membrane via interaction with phospholipid components and inhibit ice-crystal growth to reduce damage related to osmotic stress and ice crystallization. However, they may exhibit a cytotoxic effect. This review summarizes the sources and characteristics of subcellular spermatozoon cryoinjury and discusses approaches to improve outcome of fish sperm cryopreservation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40103 - Fishery
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Reviews in Aquaculture
ISSN
1753-5123
e-ISSN
—
Volume of the periodical
12
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
909-924
UT code for WoS article
000531069200019
EID of the result in the Scopus database
2-s2.0-85067402725