Seminal plasma fractions can protect common carp (Cyprinus carpio) sperm during cryopreservation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43900931" target="_blank" >RIV/60076658:12520/20:43900931 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1007/s10695-020-00805-9" target="_blank" >https://doi.org/10.1007/s10695-020-00805-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10695-020-00805-9" target="_blank" >10.1007/s10695-020-00805-9</a>
Alternative languages
Result language
angličtina
Original language name
Seminal plasma fractions can protect common carp (Cyprinus carpio) sperm during cryopreservation
Original language description
This study aimed to investigate the effect of fractionated seminal plasma on characteristics of common carp Cyprinus carpio cryopreserved sperm. Nanosep (R) centrifugal devices yielded four seminal plasma fractions with different total protein content ranging in molecular weight from less than 17 to almost 74 kDa. Each protein fraction was added to semen extender medium prior to freezing. Spermatozoon motility characteristics and DNA integrity were analyzed in supplemented and non-supplemented cryopreserved samples. The cryopreservation process strongly affected the swim-up sperm quality. Treatment with fractions 1, 2, 3, and 4 was associated with significantly higher spermatozoon motility rate and curvilinear velocity than seen in extender only, with highest values obtained with fraction 4 (78.21 +/- 2.41% and 168.05 +/- 4.46 mu m/s, respectively). Significantly less DNA damage, expressed as percent tail DNA (12.23 +/- 1.27) and olive tail moment (0.68 +/- 0.12), was recorded in fraction 4. The findings indicated that addition of fractionated seminal plasma to cryopreservation medium can preserve the quality of common carp sperm. The protective effect of each fraction varied, suggesting the presence of distinct components exerting different effects on cryopreserved sperm function.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40103 - Fishery
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Fish Physiology and Biochemistry
ISSN
0920-1742
e-ISSN
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Volume of the periodical
46
Issue of the periodical within the volume
4
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
1461-1468
UT code for WoS article
000534193800001
EID of the result in the Scopus database
2-s2.0-85085292306