Seminal plasma fractions can protect common carp (Cyprinus carpio) sperm during cryopreservation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F20%3A43934089" target="_blank" >RIV/60461373:22340/20:43934089 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12520/20:43900931

Result on the web

<a href="https://link.springer.com/article/10.1007/s10695-020-00805-9" target="_blank" >https://link.springer.com/article/10.1007/s10695-020-00805-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10695-020-00805-9" target="_blank" >10.1007/s10695-020-00805-9</a>

Result language

angličtina

Original language name

Seminal plasma fractions can protect common carp (Cyprinus carpio) sperm during cryopreservation

Original language description

This study aimed to investigate the effect of fractionated seminal plasma on characteristics of common carp Cyprinus carpio cryopreserved sperm. Nanosep® centrifugal devices yielded four seminal plasma fractions with different total protein content ranging in molecular weight from less than 17 to almost 74 kDa. Each protein fraction was added to semen extender medium prior to freezing. Spermatozoon motility characteristics and DNA integrity were analyzed in supplemented and non-supplemented cryopreserved samples. The cryopreservation process strongly affected the swim-up sperm quality. Treatment with fractions 1, 2, 3, and 4 was associated with significantly higher spermatozoon motility rate and curvilinear velocity than seen in extender only, with highest values obtained with fraction 4 (78.21 ± 2.41% and 168.05 ± 4.46 μm/s, respectively). Significantly less DNA damage, expressed as percent tail DNA (12.23 ± 1.27) and olive tail moment (0.68 ± 0.12), was recorded in fraction 4. The findings indicated that addition of fractionated seminal plasma to cryopreservation medium can preserve the quality of common carp sperm. The protective effect of each fraction varied, suggesting the presence of distinct components exerting different effects on cryopreserved sperm function. © 2020, Springer Nature B.V.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10620 - Other biological topics

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FISH PHYSIOLOGY AND BIOCHEMISTRY

ISSN

0920-1742

e-ISSN

—

Volume of the periodical

46

Issue of the periodical within the volume

4

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

1461-1468

UT code for WoS article

000534193800001

EID of the result in the Scopus database

2-s2.0-85085292306