Catalogue of stage-specific transcripts inIxodes ricinusand their potential functions during the tick life-cycle

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43901181" target="_blank" >RIV/60076658:12310/20:43901181 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/20:00539355 RIV/60077344:_____/20:00539355 RIV/00216208:11310/20:10415865

Result on the web

<a href="https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-04173-4" target="_blank" >https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-04173-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13071-020-04173-4" target="_blank" >10.1186/s13071-020-04173-4</a>

Result language

angličtina

Original language name

Catalogue of stage-specific transcripts inIxodes ricinusand their potential functions during the tick life-cycle

Original language description

Background The castor bean tickIxodes ricinusis an important vector of several clinically important diseases, whose prevalence increases with accelerating global climate changes. Characterization of a tick life-cycle is thus of great importance. However, researchers mainly focus on specific organs of fed life stages, while early development of this tick species is largely neglected. Methods In an attempt to better understand the life-cycle of this widespread arthropod parasite, we sequenced the transcriptomes of four life stages (egg, larva, nymph and adult female), including unfed and partially blood-fed individuals. To enable a more reliable identification of transcripts and their comparison in all five transcriptome libraries, we validated an improved-fit set of fiveI. ricinus-specific reference genes for internal standard normalization of our transcriptomes. Then, we mapped biological functions to transcripts identified in different life stages (clusters) to elucidate life stage-specific processes. Finally, we drew conclusions from the functional enrichment of these clusters specifically assigned to each transcriptome, also in the context of recently published transcriptomic studies in ticks. Results We found that reproduction-related transcripts are present in both fed nymphs and fed females, underlining the poorly documented importance of ovaries as moulting regulators in ticks. Additionally, we identified transposase transcripts in tick eggs suggesting elevated transposition during embryogenesis, co-activated with factors driving developmental regulation of gene expression. Our findings also highlight the importance of the regulation of energetic metabolism in tick eggs during embryonic development and glutamate metabolism in nymphs. Conclusions Our study presents novel insights into stage-specific transcriptomes ofI. ricinusand extends the current knowledge of this medically important pathogen, especially in the early phases of its development.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Parasites &amp; Vectors

ISSN

1756-3305

e-ISSN

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Volume of the periodical

13

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

19

Pages from-to

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UT code for WoS article

000542783100002

EID of the result in the Scopus database

2-s2.0-85086685493