De novo Ixodes ricinus salivary gland transcriptome analysis using two next-generation sequencing methodologies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00421247" target="_blank" >RIV/60077344:_____/13:00421247 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1096/fj.13-232140" target="_blank" >http://dx.doi.org/10.1096/fj.13-232140</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1096/fj.13-232140" target="_blank" >10.1096/fj.13-232140</a>

Result language

angličtina

Original language name

De novo Ixodes ricinus salivary gland transcriptome analysis using two next-generation sequencing methodologies

Original language description

Tick salivary gland (SG) proteins possess powerful pharmacologic properties that facilitate tick feeding and pathogen transmission. For the first time, SG transcriptomes of Ixodes ricinus, an important disease vector for humans and animals, were analyzedusing next-generation sequencing. SGs were collected from different tick life stages fed on various animal species, including cofeeding of nymphs and adults on the same host. Four cDNA samples were sequenced, discriminating tick SG transcriptomes of early- and late-feeding nymphs or adults. In total, 441,381,454 pyrosequencing reads and 67,703,183 Illumina reads were assembled into 272,220 contigs, of which 34,560 extensively annotated coding sequences are disclosed; 8686 coding sequences were submitted to GenBank. Overall, 13% of contigs were classified as secreted proteins that showed significant differences in the transcript representation among the 4 SG samples, including high numbers of sample-specific transcripts. Detailed phylog

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GAP502%2F12%2F2409" target="_blank" >GAP502/12/2409: Investigating the role of tick salivary protease inhibitors at the interface between ticks, pathogens and the vertebrate host</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FASEB Journal

ISSN

0892-6638

e-ISSN

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Volume of the periodical

27

Issue of the periodical within the volume

12

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

4745-4756

UT code for WoS article

000329999000010

EID of the result in the Scopus database

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