Gene fragmentation and RNA editing without borders: eccentric mitochondrial genomes of diplonemids

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43902478" target="_blank" >RIV/60076658:12310/20:43902478 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/20:00538265 RIV/00216208:11310/20:10411538

Result on the web

<a href="https://academic.oup.com/nar/article/48/5/2694/5699674" target="_blank" >https://academic.oup.com/nar/article/48/5/2694/5699674</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkz1215" target="_blank" >10.1093/nar/gkz1215</a>

Result language

angličtina

Original language name

Gene fragmentation and RNA editing without borders: eccentric mitochondrial genomes of diplonemids

Original language description

Diplonemids are highly abundant heterotrophic marine protists. Previous studies showed that their strikingly bloated mitochondrial genome is unique because of systematic gene fragmentation and manifold RNA editing. Here we report a comparative study of mitochondrial genome architecture, gene structure and RNA editing of six recently isolated, phylogenetically diverse diplonemid species. Mitochondria) gene fragmentation and modes of RNA editing, which include cytidine-to-uridine (C-to-U) and adenosine-to-inosine (A-to-I) substitutions and 3&apos; uridine additions (U-appendage), are conserved across diplonemids. Yet as we show here, all these features have been pushed to their extremes in the Hemistasiidae lineage. For example, Namystynia karyoxenos has its genes fragmented into more than twice as many modules than other diplonemids, with modules as short as four nucleotides. Furthermore, we detected in this group multiple A-appendage and guanosine-to-adenosine (G-to-A) substitution editing events not observed before in diplonemids and found very rarely elsewhere. With &gt;1,000 sites, C-to-U and A-to-I editing in Namystynia is nearly 10 times more frequent than in other diplonemids. The editing density of 12% in coding regions makes Namystynia&apos;s the most extensively edited transcriptome described so far. Diplonemid mitochondrial genome architecture, gene structure and post-transcriptional processes display such high complexity that they challenge all other currently known systems.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

—

Volume of the periodical

48

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

2694-2708

UT code for WoS article

000525957100041

EID of the result in the Scopus database

2-s2.0-85081077085