Nanowatt simple microcalorimetry for dynamically monitoring the defense mechanism of Paramecium caudatum

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F21%3A43903157" target="_blank" >RIV/60076658:12310/21:43903157 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/21:PU140688 RIV/60077344:_____/21:00553178 RIV/00216208:11110/21:10428050
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0924424721001059?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0924424721001059?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.sna.2021.112643" target="_blank" >10.1016/j.sna.2021.112643</a>

Result language
angličtina
Original language name
Nanowatt simple microcalorimetry for dynamically monitoring the defense mechanism of Paramecium caudatum
Original language description
Microcalorimetry has been widely used in measuring cellular metabolic heat to study bioprocesses, such as metabolism. However, it is still limited by insufficient sensitivity and system complexity, especially for the direct measurements of individual cells. Here, we present a droplet-based simple differential microcalorimetric system for determining the real-time energy balance of the ciliate protist, Paramecium caudatum. We utilized the platform to dynamically monitor its defensive behavior and measured the temperature change. Then we used heat balance equation and calculated corresponding dissipated power and energy with an ultimate resolution of approximate to 14 nW. The results showed that the defensive behavior by the ejection of the trichocysts' content consumed energy of approximate to 0.75 mJ per cell, and the dissipated power was approximate to 303.8 mu W. This differential microcalorimetric platform can be used to study the metabolic heat of protist metabolism as well as other individual cells, helping us to understand this widespread, yet little-known, biological phenomenon from a new perspective. (c) 2021 Elsevier B.V. All rights reserved.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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