Selective nuclear export of mRNAs is promoted by DRBD18 in Trypanosoma brucei
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F21%3A43903477" target="_blank" >RIV/60076658:12310/21:43903477 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/21:00554311
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1111/mmi.14773" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/mmi.14773</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/mmi.14773" target="_blank" >10.1111/mmi.14773</a>
Alternative languages
Result language
angličtina
Original language name
Selective nuclear export of mRNAs is promoted by DRBD18 in Trypanosoma brucei
Original language description
Kinetoplastids, including Trypanosoma brucei, control gene expression primarily at the posttranscriptional level. Nuclear mRNA export is an important, but understudied, step in this process. The general heterodimeric export factors, Mex67/Mtr2, function in the export of mRNAs and tRNAs in T. brucei, but RNA binding proteins (RBPs) that regulate export processes by controlling the dynamics of Mex67/Mtr2 ribonucleoprotein formation or transport have not been identified. Here, we report that DRBD18, an essential and abundant T. brucei RBP, associates with Mex67/Mtr2 in vivo, likely through its direct interaction with Mtr2. DRBD18 downregulation results in partial accumulation of poly(A)(+) mRNA in the nucleus, but has no effect on the localization of intron-containing or mature tRNAs. Comprehensive analysis of transcriptomes from whole-cell and cytosol in DRBD18 knockdown parasites demonstrates that depletion of DRBD18 leads to impairment of nuclear export of a subset of mRNAs. CLIP experiments reveal the association of DRBD18 with several of these mRNAs. Moreover, DRBD18 knockdown leads to a partial accumulation of the Mex67/Mtr2 export receptors in the nucleus. Taken together, the current study supports a model in which DRBD18 regulates the selective nuclear export of mRNAs by promoting the mobilization of export competent mRNPs to the cytosol through the nuclear pore complex.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Microbiology
ISSN
0950-382X
e-ISSN
—
Volume of the periodical
116
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
827-840
UT code for WoS article
000669291200001
EID of the result in the Scopus database
2-s2.0-85109020374