Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F09%3A00334944" target="_blank" >RIV/60077344:_____/09:00334944 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/09:00010582

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein

Original language description

All mitochondrial tRNA genes are encoded in nucleus of T. brucei and thus all tRNAs have to be imported from the cytosol. We have investigated that silencing of the cysteine desulfurase, TbNfs, the key enzyme in tRNA thiolation (s2U) and Fe-S cluster formation in vivo, has no effect on tRNA partitioning. This is especially surprising in light of a recent report suggesting that in L. tropica the Rieske Fe-S protein is an essential component of the RNA import complex. We also show that down-regulation ofthe Rieske protein by RNA interference, similar to the TbNfs knockdowns, has no effect on import. The data presented here supports: s(2)U is not a negative determinant for tRNA import; the Rieske protein is not an essential component of the import machinery, and since the Rieske protein is essential for respiration and maintenance of inner mitochondrial membrane potential, neither process plays a critical role in tRNA import.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

R N A

ISSN

1355-8382

e-ISSN

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Volume of the periodical

15

Issue of the periodical within the volume

7

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

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UT code for WoS article

000267142400017

EID of the result in the Scopus database

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