A mitochondrial cytidine deaminase is responsible for C to U editing of tRNA(Trp) to decode the UGA codon in Trypanosoma brucei

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F21%3A43903167" target="_blank" >RIV/60076658:12310/21:43903167 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/21:00554452
Result on the web
<a href="https://www.tandfonline.com/doi/full/10.1080/15476286.2021.1940445?scroll=top&needAccess=true" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/15476286.2021.1940445?scroll=top&needAccess=true</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/15476286.2021.1940445" target="_blank" >10.1080/15476286.2021.1940445</a>

Result language
angličtina
Original language name
A mitochondrial cytidine deaminase is responsible for C to U editing of tRNA(Trp) to decode the UGA codon in Trypanosoma brucei
Original language description
In kinetoplastid protists, all mitochondrial tRNAs are encoded in the nucleus and imported from the cytoplasm to maintain organellar translation. This also applies to the tryptophanyl tRNA (tRNA(Trp)) encoded by a single-copy nuclear gene, with a CCA anticodon to read UGG codon used in the cytosolic translation. Yet, in the mitochondrion it is unable to decode the UGA codon specifying tryptophan. Following mitochondrial import of tRNA(Trp), this problem is solved at the RNA level by a single C34 to U34 editing event that creates the UCA anticodon, recognizing UGA. To identify the enzyme responsible for this critical editing activity, we scrutinized the genome of Trypanosoma brucei for putative cytidine deaminases as the most likely candidates. Using RNAi silencing and poisoned primer extension, we have identified a novel deaminase enzyme, named here TbmCDAT for mitochondrial Cytidine Deaminase Acting on tRNA, which is responsible for this organelle-specific activity in T. brucei. The ablation of TbmCDAT led to the downregulation of mitochondrial protein synthesis, supporting its role in decoding the UGA tryptophan codon.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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