Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F21%3A43903617" target="_blank" >RIV/60076658:12310/21:43903617 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/21:00547077 RIV/61388971:_____/21:00547077
Result on the web
<a href="https://www.mdpi.com/1422-0067/22/12/6323" target="_blank" >https://www.mdpi.com/1422-0067/22/12/6323</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms22126323" target="_blank" >10.3390/ijms22126323</a>

Result language
angličtina
Original language name
Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages
Original language description
In vitro models are often used for studying macrophage functions, including the process of phagocytosis. The application of primary macrophages has limitations associated with the individual characteristics of animals, which can lead to insufficient standardization and higher variability of the obtained results. Immortalized cell lines do not have these disadvantages, but their responses to various signals can differ from those of the living organism. In the present study, a comparative proteomic analysis of immortalized PMJ2-R cell line and primary peritoneal macrophages isolated from C57BL/6 mice was performed. A total of 4005 proteins were identified, of which 797 were quantified. Obtained results indicate significant differences in the abundances of many proteins, including essential proteins associated with the process of phagocytosis, such as Elmo1, Gsn, Hspa8, Itgb1, Ncf2, Rac2, Rack1, Sirpa, Sod1, C3, and Msr1. These findings indicate that outcomes of studies utilizing PMJ2-R cells as a model of peritoneal macrophages should be carefully validated. All MS data are deposited in ProteomeXchange with the identifier PXD022133.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology

Project
<a href="/en/project/LTARF18021" target="_blank" >LTARF18021: Development of technologies for early detection of tick borne encephalitis, based on changes in gene expression and protein production in infected antigen-presenting cells</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)