Isolation of phages infecting the abundant freshwater Actinobacteriota order 'Ca. Nanopelagicales'
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F23%3A43906653" target="_blank" >RIV/60076658:12310/23:43906653 - isvavai.cz</a>
Result on the web
<a href="https://www.nature.com/articles/s41396-023-01400-5" target="_blank" >https://www.nature.com/articles/s41396-023-01400-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41396-023-01400-5" target="_blank" >10.1038/s41396-023-01400-5</a>
Alternative languages
Result language
angličtina
Original language name
Isolation of phages infecting the abundant freshwater Actinobacteriota order 'Ca. Nanopelagicales'
Original language description
Low-GC Actinobacteriota of the order 'Ca. Nanopelagicales' (also known as acI or hgcI clade) are abundant in freshwaters around the globe. Extensive predation pressure by phages has been assumed to be the reason for their high levels of microdiversity. So far, however, only a few metagenome-assembled phages have been proposed to infect them and no phages have been isolated. Taking advantage of recent advances in the cultivation of 'Ca. Nanopelagicales' we isolated a novel species of its genus 'Ca. Planktophila'. Using this isolate as bait, we cultivated the first two phages infecting this abundant bacterial order. Both genomes contained a whiB-like transcription factor and a RNA polymerase sigma-70 factor, which might aid in manipulating their host's metabolism. Both phages encoded a glycosyltransferase and one an anti-restriction protein, potential means to evade degradation of their DNA by nucleases present in the host genome. The two phage genomes shared only 6% of their genome with their closest relatives, with whom they form a previously uncultured family of actinophages within the Caudoviricetes. Read recruitment analyses against globally distributed metagenomes revealed the endemic distribution of this group of phages infecting 'Ca. Nanopelagicales'. The recruitment pattern against metagenomes from the isolation site and the modular distribution of shared genes between the two phages indicate high levels of horizontal gene transfer, likely mirroring the microdiversity of their host in the evolutionary arms race between host and phage.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ISME Journal
ISSN
1751-7362
e-ISSN
1751-7370
Volume of the periodical
17
Issue of the periodical within the volume
6
Country of publishing house
GB - UNITED KINGDOM
Number of pages
4
Pages from-to
943-946
UT code for WoS article
000951901900001
EID of the result in the Scopus database
2-s2.0-85150644444