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Metagenomic recovery of phage genomes of uncultured freshwater actinobacteria

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F17%3A00479538" target="_blank" >RIV/60077344:_____/17:00479538 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1038/ismej.2016.110" target="_blank" >http://dx.doi.org/10.1038/ismej.2016.110</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1038/ismej.2016.110" target="_blank" >10.1038/ismej.2016.110</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Metagenomic recovery of phage genomes of uncultured freshwater actinobacteria

  • Original language description

    Low-GC Actinobacteria are among the most abundant and widespread microbes in freshwaters and have largely resisted all cultivation efforts. Consequently, their phages have remained totally unknown. In this work, we have used deep metagenomic sequencing to assemble eight complete genomes of the first tailed phages that infect freshwater Actinobacteria. Their genomes encode the actinobacterial-specific transcription factor whiB, frequently found in mycobacteriophages and also in phages infecting marine pelagic Actinobacteria. Its presence suggests a common and widespread strategy of modulation of host transcriptional machinery upon infection via this transcriptional switch. We present evidence that some whiB-carrying phages infect the acI lineage of Actinobacteria. At least one of them encodes the ADP-ribosylating component of the widespread bacterial AB toxins family (for example, clostridial toxin). We posit that the presence of this toxin reflects a 'trojan horse' strategy, providing protection at the population level to the abundant host microbes against eukaryotic predators.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

    <a href="/en/project/GA13-00243S" target="_blank" >GA13-00243S: Unveiling life strategies of selected groups of planktonic Betaproteobacteria in relationship to carbon flow to higher trophic levels</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    The ISME Journal

  • ISSN

    1751-7362

  • e-ISSN

  • Volume of the periodical

    11

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    5

  • Pages from-to

    304-308

  • UT code for WoS article

    000394537700028

  • EID of the result in the Scopus database

    2-s2.0-84981273805