Phage-centric ecological interactions in aquatic ecosystems revealed through ultra-deep metagenomics

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F19%3A43901516" target="_blank" >RIV/60076658:12310/19:43901516 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/19:00510169

Result on the web

<a href="https://microbiomejournal.biomedcentral.com/track/pdf/10.1186/s40168-019-0752-0.pdf" target="_blank" >https://microbiomejournal.biomedcentral.com/track/pdf/10.1186/s40168-019-0752-0.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s40168-019-0752-0" target="_blank" >10.1186/s40168-019-0752-0</a>

Result language

angličtina

Original language name

Phage-centric ecological interactions in aquatic ecosystems revealed through ultra-deep metagenomics

Original language description

The persistent inertia in the ability to culture environmentally abundant microbes from aquatic ecosystems represents an obstacle in disentangling the complex web of ecological interactions spun by a diverse assortment of participants (pro- and eukaryotes and their viruses). In aquatic microbial communities, the numerically most abundant actors, the viruses, remain the most elusive, and especially in freshwaters their identities and ecology remain unknown. Here, using ultra-deep metagenomic sequencing from pelagic freshwater habitats, we recovered complete genomes of &gt; 2000 phages, including small &quot;miniphages&quot; and large &quot;megaphages&quot; infecting iconic freshwater prokaryotic lineages. For instance, abundant freshwater Actinobacteria support infection by a very broad size range of phages (13-200 Kb). We describe many phages encoding genes that likely afford protection to their host from reactive oxygen species (ROS) in the aquatic environment and in the oxidative burst in protist phagolysosomes (phage-mediated ROS defense). Spatiotemporal abundance analyses of phage genomes revealed evanescence as the primary dynamic in upper water layers, where they displayed short-lived existences. In contrast, persistence was characteristic for the deeper layers where many identical phage genomes were recovered repeatedly. Phage and host abundances corresponded closely, with distinct populations displaying preferential distributions in different seasons and depths, closely mimicking overall stratification and mixis.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

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Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microbiome

ISSN

2049-2618

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

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UT code for WoS article

000491496500001

EID of the result in the Scopus database

2-s2.0-85073606270