13CO2 labelling as a tool for elucidating the mechanism of cuticle development: a case of Clusia rosea
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F23%3A43907143" target="_blank" >RIV/60076658:12310/23:43907143 - isvavai.cz</a>
Result on the web
<a href="https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.18716" target="_blank" >https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.18716</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/nph.18716" target="_blank" >10.1111/nph.18716</a>
Alternative languages
Result language
angličtina
Original language name
13CO2 labelling as a tool for elucidating the mechanism of cuticle development: a case of Clusia rosea
Original language description
The plant cuticle is an important plant-atmosphere boundary, the synthesis and maintenance of which represents a significant metabolic cost. Only limited information regarding cuticle dynamics is available.We determined the composition and dynamics of Clusia rosea cuticular waxes and matrix using (CO2)-C-13 labelling, compound-specific and bulk isotope ratio mass spectrometry. Collodion was used for wax collection; gas exchange techniques to test for any collodion effects on living leaves.Cutin matrix (MX) area density did not vary between young and mature leaves and between leaf sides. Only young leaves incorporated new carbon into their MX. Collodion-based sampling discriminated between epicuticular (EW) and intracuticular wax (IW) effectively. Epicuticular differed in composition from IW. The newly synthetised wax was deposited in IW first and later in EW. Both young and mature leaves synthetised IW and EW. The faster dynamics in young leaves were due to lower wax coverage, not a faster synthesis rate. Longer-chain alkanes were deposited preferentially on the abaxial, stomatous leaf side, producing differences between leaf sides in wax composition.We introduce a new, sensitive isotope labelling method and demonstrate that cuticular wax is renewed during leaf ontogeny of C. rosea. We discuss the ecophysiological significance of the new insights.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
New Phytologist
ISSN
0028-646X
e-ISSN
1469-8137
Volume of the periodical
238
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
14
Pages from-to
202-215
UT code for WoS article
000916891200001
EID of the result in the Scopus database
2-s2.0-85147296518