Amphistomy: stomata patterning inferred from 13C content and leaf-side-specific deposition of epicuticular wax

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F24%3A43908431" target="_blank" >RIV/60076658:12310/24:43908431 - isvavai.cz</a>

Result on the web

<a href="https://academic.oup.com/aob/article/134/3/437/7688001?login=true" target="_blank" >https://academic.oup.com/aob/article/134/3/437/7688001?login=true</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/aob/mcae082" target="_blank" >10.1093/aob/mcae082</a>

Result language

angličtina

Original language name

Amphistomy: stomata patterning inferred from 13C content and leaf-side-specific deposition of epicuticular wax

Original language description

Background and Aims: The benefits and costs of amphistomy (AS) vs. hypostomy (HS) are not fully understood. Here, we quantify benefits of access of CO2 through stomata on the upper (adaxial) leaf surface, using C-13 abundance in the adaxial and abaxial epicuticular wax. Additionally, a relationship between the distribution of stomata and epicuticular wax on the opposite leaf sides is studied. Methods: We suggest that the C-13 content of long-chain aliphatic compounds of cuticular wax records the leaf internal CO2 concentration in chloroplasts adjacent to the adaxial and abaxial epidermes. This unique property stems from: (1) wax synthesis being located exclusively in epidermal cells; and (2) ongoing wax renewal over the whole leaf lifespan. Compound-specific and bulk wax C-13 abundance (delta) was related to amphistomy level (ASL; as a fraction of adaxial in all stomata) of four AS and five HS species grown under various levels of irradiance. The isotopic polarity of epicuticular wax, i.e. the difference in abaxial and adaxial delta (delta(ab) - delta(ad)), was used to calculate the leaf dorsiventral CO2 gradient. Leaf-side-specific epicuticular wax deposition (amphiwaxy level) was estimated and related to ASL. Key Results: In HS species, the CO2 concentration in the adaxial epidermis was lower than in the abaxial one, independently of light conditions. In AS leaves grown in high-light and low-light conditions, the isotopic polarity and CO2 gradient varied in parallel with ASL. The AS leaves grown in high-light conditions increased ASL compared with low light, and delta(ab) - delta(ad) approached near-zero values. Changes in ASL occurred concomitantly with changes in amphiwaxy level. Conclusions: Leaf wax isotopic polarity is a newly identified leaf trait, distinguishing between hypo- and amphistomatous species and indicating that increased ASL in sun-exposed AS leaves reduces the CO2 gradient across the leaf mesophyll. Stomata and epicuticular wax deposition follow similar leaf-side patterning.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10611 - Plant sciences, botany

Project

<a href="/en/project/GA18-14704S" target="_blank" >GA18-14704S: Stomata: coordination of development, evolution of CO2 sensing and carbon acquisition</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Annals of Botany

ISSN

0305-7364

e-ISSN

1095-8290

Volume of the periodical

134

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

18

Pages from-to

437-454

UT code for WoS article

001253850500001

EID of the result in the Scopus database

2-s2.0-85202081716