Partitioning of mesophyll conductance for CO2 into intercellular and cellular components using carbon isotope composition of cuticles from opposite leaf sides

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F19%3A43899487" target="_blank" >RIV/60076658:12310/19:43899487 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/19:00507526 RIV/61389030:_____/19:00507526 RIV/61989100:27710/19:10242266

Result on the web

<a href="https://link.springer.com/content/pdf/10.1007%2Fs11120-019-00628-7.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007%2Fs11120-019-00628-7.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11120-019-00628-7" target="_blank" >10.1007/s11120-019-00628-7</a>

Result language

angličtina

Original language name

Partitioning of mesophyll conductance for CO2 into intercellular and cellular components using carbon isotope composition of cuticles from opposite leaf sides

Original language description

We suggest a new technique for estimating the relative drawdown of CO2 concentration (c) in the intercellular air space (IAS) across hypostomatous leaves (expressed as the ratio c(d)/c(b), where the indexes d and b denote the adaxial and abaxial edges, respectively, of IAS), based on the carbon isotope composition (delta C-13) of leaf cuticular membranes (CMs), cuticular waxes (WXs) or epicuticular waxes (EWXs) isolated from opposite leaf sides. The relative drawdown in the intracellular liquid phase (i.e., the ratio c(c)/c(bd), where c(c) and c(bd) stand for mean CO2 concentrations in chloroplasts and in the IAS), the fraction of intercellular resistance in the total mesophyll resistance (r(IAS)/r(m)), leaf thickness, and leaf mass per area (LMA) were also assessed. We show in a conceptual model that the upper (adaxial) side of a hypostomatous leaf should be enriched in C-13 compared to the lower (abaxial) side. CM, WX, and/or EWX isolated from 40 hypostomatous C-3 species were C-13 depleted relative to bulk leaf tissue by 2.01-2.85 parts per thousand. The difference in delta C-13 between the abaxial and adaxial leaf sides (delta C-13(AB)-C-13(AD), Delta(b-d)), ranged from -2.22 to +0.71 parts per thousand (-0.09 +/- 0.54 parts per thousand, mean +/- SD) in CM and from -7.95 to 0.89 parts per thousand (-1.17 +/- 1.40 parts per thousand) in WX. In contrast, two tested amphistomatous species showed no significant Delta(b-d) difference in WX. Delta(b-d) correlated negatively with LMA and leaf thickness of hypostomatous leaves, which indicates that the mesophyll air space imposes a non-negligible resistance to CO2 diffusion. delta C-13 of EWX and 30-C aldehyde in WX reveal a stronger CO2 drawdown than bulk WX or CM. Mean values of c(d)/c(b) and c(c)/c(bd) were 0.90 +/- 0.12 and 0.66 +/- 0.11, respectively, across 14 investigated species in which wax was isolated and analyzed. The diffusion resistance of IAS contributed 20 +/- 14% to total mesophyll resistance and reflects species-specific and environmentally-induced differences in leaf functional anatomy.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Photosynthesis Research

ISSN

0166-8595

e-ISSN

—

Volume of the periodical

141

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

19

Pages from-to

33-51

UT code for WoS article

000474427900003

EID of the result in the Scopus database

2-s2.0-85062465091