Counting sedimented Betula pollen grains by gas chromatography coupled with mass spectrometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F25%3A43909991" target="_blank" >RIV/60076658:12310/25:43909991 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007/s10453-025-09855-z?utm_source=getftr&utm_medium=getftr&utm_campaign=getftr_pilot&getft_integrator=clarivate" target="_blank" >https://link.springer.com/article/10.1007/s10453-025-09855-z?utm_source=getftr&utm_medium=getftr&utm_campaign=getftr_pilot&getft_integrator=clarivate</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10453-025-09855-z" target="_blank" >10.1007/s10453-025-09855-z</a>

Result language

angličtina

Original language name

Counting sedimented Betula pollen grains by gas chromatography coupled with mass spectrometry

Original language description

Birch pollen grains (BPGs) are major aeroallergens in Europe, causing allergies in millions of people. Although background concentrations are provided by stationary pollen monitoring stations, they do not accurately reflect personal exposure. Knowing personal exposure makes it possible to establish a link between inhaled pollen grains and symptoms. Characterizing personal exposure to pollen using portable devices is challenging and requires time-consuming visual counting of pollen grains. We have developed a method for counting sedimented BPGs based on easy sampling using a handheld hoover and automated analysis by gas chromatography-mass spectrometry (GC-MS). This work is a feasibility study on the use of a lipid tracer (heptacosane) for the mass quantification of sedimented BPGs in outdoor and indoor environments. Before a lipid tracer could be used for the determination of BPGs, we ensured that the variability of total lipid mass was low (around 17%) for pollen samples from various geographical origins and for several pollen seasons. The limit of quantification of sedimented BPGs by GC-MS was estimated to be 100 mu g (equivalent to 16,000 BPGs), i.e. about 1.6 BPG cm-2 for a sampled surface of 1 m2. This method of assessing individual exposure was implemented during the birch pollen season indoors (between 70 and 225 sedimented BPGs per cm2) and outdoors, directly on the ground under a birch tree (over 6600 BPGs per cm2). This method of counting sedimented pollen grains is suitable for large sample series, and the data obtained could be used as an indicator of individual exposure to indoor air pollen in a large number of patients as part of epidemiological surveys.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10511 - Environmental sciences (social aspects to be 5.7)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

AEROBIOLOGIA

ISSN

0393-5965

e-ISSN

1573-3025

Volume of the periodical

41

Issue of the periodical within the volume

2

Country of publishing house

IT - ITALY

Number of pages

12

Pages from-to

333-344

UT code for WoS article

001467007700001

EID of the result in the Scopus database

2-s2.0-105002611574