Stick together: isolation and characterization of exopolysaccharides producing psychrotolerant bacteria from Alaskan permafrost soils

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F25%3A43910598" target="_blank" >RIV/60076658:12310/25:43910598 - isvavai.cz</a>

Result on the web

<a href="http://chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/https://link.springer.com/content/pdf/10.1007/s00300-025-03435-0.pdf?utm_source=clarivate&getft_integrator=clarivate" target="_blank" >http://chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/https://link.springer.com/content/pdf/10.1007/s00300-025-03435-0.pdf?utm_source=clarivate&getft_integrator=clarivate</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00300-025-03435-0" target="_blank" >10.1007/s00300-025-03435-0</a>

Result language

angličtina

Original language name

Stick together: isolation and characterization of exopolysaccharides producing psychrotolerant bacteria from Alaskan permafrost soils

Original language description

Bacterial exopolysaccharides (EPS) act as natural biopolymers that bind soil particles together, promoting structural stability and creating protective microenvironments for microbial survival. This study aimed to isolate and characterize potential EPS-producing bacteria from the active layer of two degraded permafrost soils with different hydrological landscapes, and from non-degraded permafrost soil. A total of 64 bacterial isolates were obtained, representing three phyla: Firmicutes, Actinomycetota, and Pseudomonadota. EPS production was assessed by determining the polysaccharide content measured as glucose equivalent, and 26 bacterial isolates were identified as potential EPS producers. Among the bacterial isolates, Curtobacterium oceanosedimentum, Frigoribacterium faeni, Streptomyces strains, Neobacillus bataviensis, and Mesobacillus subterraneus exhibited high polysaccharide concentrations. Uronic acids were present in EPS produced by C. oceanosedimentum and N. bataviensis, while amino sugars were identified in EPS from isolates of Bacillus, Streptomyces, Luteimonas, and Phyllobacterium. Based on 16S rRNA gene sequence similarities, the relative proportion of taxa associated with EPS-producing bacterial isolates such as Bacillus, Peribacillus, and Streptomyces was higher in the dry site, while Neobacillus, Pseudarthrobacter, and Microbacterium were more abundant in the wet and intact sites. This study suggests that EPS production with diverse carbohydrate compositions primarily promotes structural stability in degrading permafrost soils by binding soil particles together and forming protective microenvironments. Additionally, EPS may contribute to nutrient retention and microbial protection under fluctuating environmental conditions, complementing their primary role in soil stability.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GC20-21259J" target="_blank" >GC20-21259J: CRYOVULCAN - Vulnerability of carbon in Cryosols – substrate-microorganisms-aggregate interactions</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Polar Biology

ISSN

0722-4060

e-ISSN

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Volume of the periodical

48

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

15

Pages from-to

nestránkováno

UT code for WoS article

001621649200003

EID of the result in the Scopus database

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