Isolation and identification of bacteria isolates from arsenic contaminated anthroposoils

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27350%2F14%3A86089567" target="_blank" >RIV/61989100:27350/14:86089567 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.5593/SGEM2014/B51/S20.054" target="_blank" >http://dx.doi.org/10.5593/SGEM2014/B51/S20.054</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5593/SGEM2014/B51/S20.054" target="_blank" >10.5593/SGEM2014/B51/S20.054</a>

Result language

angličtina

Original language name

Isolation and identification of bacteria isolates from arsenic contaminated anthroposoils

Original language description

This study aims to isolate and identify autochthonous bacteria from arseniccontaminated soils collected in the locality of Zemianske Kostoľany. After the coal ash pond spill in 1965, soils, sediments, and ground water were all significantly contaminated with arsenic exceeding 1500 ppm and with other potentially toxic elements. Firstly, sixty-three morphologically different bacterial isolates were obtained from samples of contaminated substrates. Consequently, identification of 16S rRNA sequences showed identities to genus Pseudomonas, Rhodococcus, Bacillus, Streptomyces and Chryseobacterium. Out of these, Pseudomonas was the genus of the greatest proportion of isolates in the studied ashy-soil substrates (62.5 %). The genus was represented by the species Pseudomonas sp., P. chlororaphis, P. putida, P. baetica and P. reinekei. The results showed that the genus Bacillus had the second highest abundance (20.83 %) and was represented by species B. cereus and B. pumilus. Two isolates of the genus Bacillus were classified as uncultured soil bacterium clone. There were further recorded two isolates of the genus Chryseobacterium. The genus Streptomyces and Rhodococcus were represented by only one species. Last but not least, 20 % of isolates were not identified to their genus. Results of studied bacterial species diversity, which was able to adapt to living in contaminated environment, are the basis for the application of selected indigenous bacterial species in bioleaching process as one of the potential methods for bioremediation of arsenic-contaminated soils.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

DH - Mining industry including coal mining and processing

OECD FORD branch

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Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

SGEM 2014 : GeoConference on Nano, Bio and Green-Technologies for a Sustainable Future : 17-26, June, 2014, Albena, Bulgaria : Volume I, Micro and Nano Technologies Advances in Biotechnogy

ISBN

978-619-7105-17-9

ISSN

1314-2704

e-ISSN

—

Number of pages

6

Pages from-to

399-404

Publisher name

STEF92 Technology Ltd.

Place of publication

Sofia

Event location

Albena

Event date

Jun 17, 2014

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000370816200054