Preliminary Studies on Cryopreservation of Common Tench (Tinca tinca) Embryos (Work in Progress)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F09%3A00010277" target="_blank" >RIV/60076658:12520/09:00010277 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Preliminary Studies on Cryopreservation of Common Tench (Tinca tinca) Embryos (Work in Progress)
Original language description
Vitrification could provide a promising tool for the cryopreservation of fish embryos. However, to achieve cryopreservation using vitrification, chilling sensitivity and cryoprotectants toxicity were determined using tench embryos at four developmental stages (11, 17, 23 and 29 h). Embryos treated with alcalase (2 ml/998 ml, 2 min at 22 degrees C) were exposed to chilling with/without warming. Other embryos were exposed to methanol and glycerol at the concentration of 10% and 20% for periods of 20 min.At last, embryos were incubated at special incubator cages where hatching rates were counted. Regarding chilling sensitivity and exposure to chilling followed by warming, the hatching rates of embryos decreased significantly (p < 0.001) after exposure to0 degrees C at all developmental stages except the 29-h stage compared with the controls. The embryo stage most sensitive to chilling was 11-h stage. The 29-h stage exhibited the least sensitivity to low temperature while 17-h and 23-h s
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GL - Fishery
OECD FORD branch
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Project
<a href="/en/project/QH82119" target="_blank" >QH82119: Research of sperm and embryos cryopreservation</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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