Stage-dependent differences in RNA composition and content affect the outcome of expression profiling in roach (Rutilus rutilus) ovary
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F11%3A43873477" target="_blank" >RIV/60076658:12520/11:43873477 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.cbpa.2011.02.007" target="_blank" >http://dx.doi.org/10.1016/j.cbpa.2011.02.007</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.cbpa.2011.02.007" target="_blank" >10.1016/j.cbpa.2011.02.007</a>
Alternative languages
Result language
angličtina
Original language name
Stage-dependent differences in RNA composition and content affect the outcome of expression profiling in roach (Rutilus rutilus) ovary
Original language description
The influence of changing composition and content of RNA on the results of expression profiling was studied in the ovaries of roach (Rutilus rutilus). The highest total RNA yield was detected in the primary growth (pg) and early cortical alveolus (eca) stages. The total RNA yield gradually decreased through the late cortical alveolus and late vitellogenic stages. In the pg and eca stages, total RNA was characterized by a low percentage of 18S and 28S rRNA and a high content of smaller-sized RNAs, opposite pattern was observed in later stages. The ratio mRNA/total RNA was highest at the pg stage but decreased significantly in later stages. When total RNA was used for RT, the shift in the mRNA/total RNA ratio influenced the results of qPCR of four commonly used reference and two target genes (TG). Therefore, the expression of TG should be related to the mRNA pool using the same input of either mRNA to RT or cDNA to qPCR. Gene expression could be also related to tissue-specific RNA yield
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ED2.1.00%2F01.0024" target="_blank" >ED2.1.00/01.0024: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology
ISSN
1095-6433
e-ISSN
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Volume of the periodical
159
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
141-149
UT code for WoS article
000290195500006
EID of the result in the Scopus database
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