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Stage-dependent differences in RNA composition and content affect the outcome of expression profiling in roach (Rutilus rutilus) ovary

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F11%3A43873477" target="_blank" >RIV/60076658:12520/11:43873477 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1016/j.cbpa.2011.02.007" target="_blank" >http://dx.doi.org/10.1016/j.cbpa.2011.02.007</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.cbpa.2011.02.007" target="_blank" >10.1016/j.cbpa.2011.02.007</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Stage-dependent differences in RNA composition and content affect the outcome of expression profiling in roach (Rutilus rutilus) ovary

  • Original language description

    The influence of changing composition and content of RNA on the results of expression profiling was studied in the ovaries of roach (Rutilus rutilus). The highest total RNA yield was detected in the primary growth (pg) and early cortical alveolus (eca) stages. The total RNA yield gradually decreased through the late cortical alveolus and late vitellogenic stages. In the pg and eca stages, total RNA was characterized by a low percentage of 18S and 28S rRNA and a high content of smaller-sized RNAs, opposite pattern was observed in later stages. The ratio mRNA/total RNA was highest at the pg stage but decreased significantly in later stages. When total RNA was used for RT, the shift in the mRNA/total RNA ratio influenced the results of qPCR of four commonly used reference and two target genes (TG). Therefore, the expression of TG should be related to the mRNA pool using the same input of either mRNA to RT or cDNA to qPCR. Gene expression could be also related to tissue-specific RNA yield

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/ED2.1.00%2F01.0024" target="_blank" >ED2.1.00/01.0024: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2011

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology

  • ISSN

    1095-6433

  • e-ISSN

  • Volume of the periodical

    159

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    9

  • Pages from-to

    141-149

  • UT code for WoS article

    000290195500006

  • EID of the result in the Scopus database