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Spatial expression profiles in the Xenopus laevis oocytes measured with qPCR tomography

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F10%3A10080983" target="_blank" >RIV/00216208:11310/10:10080983 - isvavai.cz</a>

  • Alternative codes found

    RIV/86652036:_____/10:00346483

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Spatial expression profiles in the Xenopus laevis oocytes measured with qPCR tomography

  • Original language description

    qPCR tomography was developed to study mRNA localization in complex biological samples that are embedded and cryo-sectioned. After total RNA extraction and reverse transcription, the spatial profiles of mRNAs and other functional RNAs were determined byqPCR. The Xenopus laevis oocyte was selected as model, because of its large size (more than 1 mm) and large amount of total RNA ( 5 mýg). Fifteen sections along the animal-vegetal axis were cut and prepared for quantification of 31 RNA targets using thehigh-throughput real-time RT-PCR (qPCR) BioMark? platform. mRNAs were found to have two localization patterns, animal/central or vegetal. Because of the high resolution in sectioning, it was possible to distinguish two subgroups of the vegetal gene patterns: germ plasm determinant pattern and profile of other vegetal genes.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2010

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Methods

  • ISSN

    1046-2023

  • e-ISSN

  • Volume of the periodical

    51

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    5

  • Pages from-to

  • UT code for WoS article

    000277953300013

  • EID of the result in the Scopus database