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Use of Feulgen image analysis densitometry to study the effect of genome size on nuclear size in polyploid sturgeons

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F12%3A43883453" target="_blank" >RIV/60076658:12520/12:43883453 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1111/j.1439-0426.2012.02021.x" target="_blank" >http://dx.doi.org/10.1111/j.1439-0426.2012.02021.x</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1111/j.1439-0426.2012.02021.x" target="_blank" >10.1111/j.1439-0426.2012.02021.x</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Use of Feulgen image analysis densitometry to study the effect of genome size on nuclear size in polyploid sturgeons

  • Original language description

    Feulgen image analysis densitometry (FIA) and image cytometry were used to study the relationship between the DNA content (pgDNA nucleus-1) and nuclear area (mu m2) in blood smears of evolutionary tetraploid (4n) sterlet (Acipenser ruthenus) and stellatesturgeon (A. stellatus); evolutionary octaploid (8n) Siberian sturgeon (A. baerii) and Russian sturgeon (A. gueldenstaedtii); hexaploid (6n) and decaploid (10n) fish found within A. baerii stock; and A. baerii and A. gueldenstaedtii exhibiting dodecaploidy (12n). Standards used for FIA were blood smears of chicken (Gallus gallus domesticus; 2.5 pgDNA nucleus-1) and diploid and induced triploid tench, Tinca tinca (2.04 and 3.1 pgDNA nucleus-1, respectively). All ploidy levels were first verified by means of flow cytometry. Species of the same ploidy level, however differing in their DNA content, exhibited a similar mean erythrocyte nuclear area, as could be demonstrated on A. ruthenus and A. stellatus (19.27 and 19.79 mu m2, respectivel

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    GL - Fishery

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Applied Ichthyology

  • ISSN

    0175-8659

  • e-ISSN

  • Volume of the periodical

    28

  • Issue of the periodical within the volume

    5

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    5

  • Pages from-to

    704-708

  • UT code for WoS article

    000308878000005

  • EID of the result in the Scopus database