Use of Feulgen image analysis densitometry to study the effect of genome size on nuclear size in polyploid sturgeons

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F12%3A43883453" target="_blank" >RIV/60076658:12520/12:43883453 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1111/j.1439-0426.2012.02021.x" target="_blank" >http://dx.doi.org/10.1111/j.1439-0426.2012.02021.x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1439-0426.2012.02021.x" target="_blank" >10.1111/j.1439-0426.2012.02021.x</a>

Result language

angličtina

Original language name

Use of Feulgen image analysis densitometry to study the effect of genome size on nuclear size in polyploid sturgeons

Original language description

Feulgen image analysis densitometry (FIA) and image cytometry were used to study the relationship between the DNA content (pgDNA nucleus-1) and nuclear area (mu m2) in blood smears of evolutionary tetraploid (4n) sterlet (Acipenser ruthenus) and stellatesturgeon (A. stellatus); evolutionary octaploid (8n) Siberian sturgeon (A. baerii) and Russian sturgeon (A. gueldenstaedtii); hexaploid (6n) and decaploid (10n) fish found within A. baerii stock; and A. baerii and A. gueldenstaedtii exhibiting dodecaploidy (12n). Standards used for FIA were blood smears of chicken (Gallus gallus domesticus; 2.5 pgDNA nucleus-1) and diploid and induced triploid tench, Tinca tinca (2.04 and 3.1 pgDNA nucleus-1, respectively). All ploidy levels were first verified by means of flow cytometry. Species of the same ploidy level, however differing in their DNA content, exhibited a similar mean erythrocyte nuclear area, as could be demonstrated on A. ruthenus and A. stellatus (19.27 and 19.79 mu m2, respectivel

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GL - Fishery

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Ichthyology

ISSN

0175-8659

e-ISSN

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Volume of the periodical

28

Issue of the periodical within the volume

5

Country of publishing house

DE - GERMANY

Number of pages

5

Pages from-to

704-708

UT code for WoS article

000308878000005

EID of the result in the Scopus database

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