Use of diploid and triploid tench (Tinca tinca) blood as standards for genome size measurements

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F14%3A43886833" target="_blank" >RIV/60076658:12520/14:43886833 - isvavai.cz</a>
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1111/jai.12422/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/jai.12422/abstract</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jai.12422" target="_blank" >10.1111/jai.12422</a>

Result language
angličtina
Original language name
Use of diploid and triploid tench (Tinca tinca) blood as standards for genome size measurements
Original language description
The ploidy level of 10 diploid and 10 induced triploid tench, Tinca tinca, was verified using flow cytometry to determine relative DNA content of DAPI-stained erythrocyte nuclei. The C-value (haploid nuclear DNA content; pgDNAnucleus-1) of these same individuals was determined by means of Feulgen image analysis densitometry, in comparison to the chicken (Gallus gallus domesticus; 1.25pg P{0.05) standard, using three different approaches. Highly similar mean C-values were obtained, thus confirming the possibility of using tench blood as a frequently used standard in European pond aquaculture for ploidy determination in fishes.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GL - Fishery
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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