Optimization of sperm irradiation protocol for induced gynogenesis in Siberian sturgeon, Acipenser baerii
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F14%3A43886835" target="_blank" >RIV/60076658:12520/14:43886835 - isvavai.cz</a>
Result on the web
<a href="http://link.springer.com/article/10.1007%2Fs10499-013-9658-1" target="_blank" >http://link.springer.com/article/10.1007%2Fs10499-013-9658-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10499-013-9658-1" target="_blank" >10.1007/s10499-013-9658-1</a>
Alternative languages
Result language
angličtina
Original language name
Optimization of sperm irradiation protocol for induced gynogenesis in Siberian sturgeon, Acipenser baerii
Original language description
The great diversity of optimal UV irradiation doses are used for DNA inactivation in fish sperm forcing authors to repeat optimization of irradiation treatment every time. Analysis of sperm UV irradiation protocol for induction of gynogenesis showed theimportance of sperm UV light absorption estimations. The UV absorption investigation in Siberian sturgeon sperm showed average extinction coefficient 7.69 x 10(-8) +/- A 1.04 x 10(-8) cm(2). It is resulted in high heterogeneity of UV irradiation of undiluted sperm samples. Therefore, it is strongly suggested to specify doses only with defined concentration of spermatozoa; otherwise, the difference in absorbance level between samples can bring a significant error to optimal UV dose estimation. This was confirmed by UV-irradiated sperm motility investigation. Results of motility investigation of UV-irradiated sperm revealed high sensitivity of Siberian sturgeon spermatozoa motion mechanisms to UV irradiation, with complete loss of motilit
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Aquaculture International
ISSN
0967-6120
e-ISSN
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Volume of the periodical
22
Issue of the periodical within the volume
2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
485-495
UT code for WoS article
000332315700012
EID of the result in the Scopus database
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